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The full Yeasen knowledge base, organised by two-level categories and six content types, with source links on every article.

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Techniques一文全面解析NGS接头的奥秘

如何在茫茫数据中找到对应样本匹配的数据呢?人们想到了一种在文库构建时在接头上添加“接头暗号”的方法,在测序完成之后根据“接头暗号”对样本进行分离。这里的接头暗号就是样本标签“Index/Barcode”。本文将以建库过程中要添加的接头为核心,对其进行全面介绍。

📂 接头 👁 17,751 🗓 2026-10-12

Techniques为什么非要检测rcAAV,rcAAV到底有什么风险?

翌圣生物自主研发了复制型腺相关病毒血清型2 (rcAAV2)检测试剂盒,基于荧光探针定量PCR原理,采用qPCR法分别检测rAAV末端重复序列(ITR)(即参考基因Reference)和rcAAV的连接序列(即靶基因Target),实现对rAAV和rcAAV的同时定量检测。还研发了与之配套使用的宿主细胞残留DNA样本前处理试剂盒,以及配套的自动化核酸提取仪器

📂 基因编辑 👁 17,943 🗓 2026-10-12
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