Article Detail
Buying Guides普通PCR产品选购指南
📂 PCR系列 Type ID 5001
普通PCR产品选购指南
Source: Yeasen Biotech | Original: https://www.yeasen.com/solutiondetail/1162 ↗
产品系列 | 快速PCR系列 | 经典普通PCR系列 | 长片段PCR系列 | 小鼠基因型鉴定 | |||
产品货号 | |||||||
特点 | |||||||
扩增长度 | ≤10 kb 细菌和真菌(菌落、菌液) | ≤8 kb gDNA | ≤5 kb | ≤25 kb gDNA | ≤4 kb | ||
延伸时间(/kb) | 1-10 s(3 kb以内菌P快至1 s/kb;10 kb λDNA快至1 s/kb) | 1-10 s | 30 s | 30 s | 30 s | ||
产物末端结构 | 3’-dA | 3’-dA | 3'-dA | 3'-dA | 3‘-dA | ||
退火温度 | 60℃ | 60-(1~2)℃ | Tm-(2~5)℃ | 68℃ | Tm-(2~5)℃ | ||
GC兼容范围 | 30-70% | 30-70% | 40-70% | 30-70% | 30-70% | ||
5‘-3’外切酶活性 | O | O | O | O | O | ||
应用 | |||||||
菌液PCR | O | O | O | O | O | ||
基因鉴定 | O | O | O | O | O | ||
多重PCR | O(3-4重) | ||||||
其他特点 | |||||||
电泳指示剂 | 紫红色 | 蓝色 | 蓝色 | 无色 | 无色 | 绿色 | 蓝色 |
热启动 | O | O | O | O | |||
预混液/试剂盒 | Mix | Mix | Mix | Mix | Mix+dNTP+MgSO4 | Mix | |
注:如需进行PAGE电泳,可以选购10160ES。
Source
Source:
Yeasen Biotech
Original URL:
https://www.yeasen.com/solutiondetail/1162
Source ID: 1162
Content is compiled from Yeasen Biotech public materials; all rights belong to the original author.
Products CoveredFrom source article
IMG
2× Hieff® Robust PCR Master Mix (No Dye)
Not listed yet IMG 2× Hieff® PCR Master Mix for PAGE
Not listed yet IMG 2× Hieff® PCR Master Mix (No Dye)
Not listed yet IMG 2× Hieff® Robust PCR Master Mix (With Dy...
Not listed yet IMG 2× Hieff® PCR Master Mix (With Dye)
Not listed yet IMG 2× Hieff Canace® AdvanceFast PCR Master ...
Not listed yet IMG Hieff Canace® Gold High-Fidelity DNA Pol...
Not listed yet IMG 2× Hieff Canace® Gold PCR Master Mix 高保真...
Not listed yet IMG Hieff Canace®Veritas Long PCR Master Mix...
Not listed yet IMG Hieff Canace® Plus High-Fidelity DNA Pol...
Not listed yet IMG 2× Hieff Canace® AdvanceFast PCR Master ...
Not listed yet IMG 2× Hieff Canace® Plus PCR Master Mix (Wi...
Not listed yet IMG Hieff Canace®Veritas Long PCR Master Mix...
Not listed yet IMG 2× Hieff Canace® PCR Master Mix 高保真酶预...
Not listed yet
Not listed yet IMG 2× Hieff® PCR Master Mix for PAGE
Not listed yet IMG 2× Hieff® PCR Master Mix (No Dye)
Not listed yet IMG 2× Hieff® Robust PCR Master Mix (With Dy...
Not listed yet IMG 2× Hieff® PCR Master Mix (With Dye)
Not listed yet IMG 2× Hieff Canace® AdvanceFast PCR Master ...
Not listed yet IMG Hieff Canace® Gold High-Fidelity DNA Pol...
Not listed yet IMG 2× Hieff Canace® Gold PCR Master Mix 高保真...
Not listed yet IMG Hieff Canace®Veritas Long PCR Master Mix...
Not listed yet IMG Hieff Canace® Plus High-Fidelity DNA Pol...
Not listed yet IMG 2× Hieff Canace® AdvanceFast PCR Master ...
Not listed yet IMG 2× Hieff Canace® Plus PCR Master Mix (Wi...
Not listed yet IMG Hieff Canace®Veritas Long PCR Master Mix...
Not listed yet IMG 2× Hieff Canace® PCR Master Mix 高保真酶预...
Not listed yet