技术讲解细胞转染注意事项,你都get了吗?(下)
细胞转染注意事项,你都get了吗?(下)
细胞转染实验从开始设计到最终的效果检测,可以由6个过程组成:核酸准备、试剂选择、细胞铺板、转染操作、细胞培养、效果检测。每一步都会影响最终的结果,均存在不同的注意事项。上个月小翌分享了前3个过程的注意事项:

本期分享后面3个过程的注意事项:
转染操作
1.条件摸索与优化:
2.无血清配置复合物:
3.孵育时间:
4.抗生素影响:
1.优良的培养试剂:
2.是否换液:
1.检测方法的选择:
2.检测时间的选择:
3.稳转筛选:
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翌圣生物转染试剂产品
应用场景 | 名称 | 货号 | 规格 |
细胞类型:贴壁/悬浮 核酸类型:DNA、siRNA | 40802ES02 | 0.5 mL | |
40802ES03 | 1.0 mL | ||
40802ES08 | 5×1mL | ||
细胞类型:贴壁/悬浮 | 40803ES70 | 200 T | |
用途:病毒感染、DNA转染 | 40804ES76 | 500 μL | |
40804ES86 | 5×500 μL | ||
细胞类型:悬浮 | 40805ES02 | 0.5 mL | |
40805ES03 | 1.0 mL | ||
40805ES08 | 5×1 mL | ||
细胞类型:贴壁/悬浮 | 40806ES01 | 0.1 mL | |
40806ES02 | 0.5 mL | ||
40806ES03 | 1.0 mL | ||
细胞类型:贴壁/悬浮 | 40808ES02 | 0.5 mL | |
40808ES03 | 1 mL | ||
40808ES08 | 5×1 mL | ||
细胞类型:贴壁/悬浮 | 40809ES01 | 0.1 mL | |
40809ES03 | 1 mL | ||
细胞类型:贴壁/悬浮 | 40815ES03 | 1 g | |
40815ES08 | 5×1 g | ||
细胞类型:贴壁/悬浮 | 40816ES02 | 100 mg | |
40816ES03 | 1 g | ||
细胞类型:293 | 40820ES04 | 1.5 mL | |
40820ES10 | 10 mL | ||
40820ES60 | 100 mL | ||
细胞类型:293 核酸类型:DNA 用途:AAV/LV载体大规模生产 | 40821ES10 | 10 mL | |
40821ES60 | 100 mL | ||
40821ES80 | 1 L |
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