生物制药 - Yeasen Knowledge Base

Case Studies细胞转染 | HK2人肾近曲小管上皮细胞siRNA转染案例

实验所用转染试剂试剂名称货号Hieff Trans®RNAiBoost Transfection Reagent转染试剂40807ES细胞培养与传代（T25）润洗：吸弃旧培养基，加入3-4 mL常温PBS轻晃润洗10-20秒后吸弃。消化：加入1 mL胰酶润湿瓶底，37°C孵育消化。吹落：待细胞间隙变大、形态变圆时，直接用胰酶轻轻吹打下细胞。终止：加入2-3

📂 细胞转染 👁 11,291 🗓 2026-10-09

Source: Yeasen Biotech·https://www.yeasen.com/solutiondetail/2034524178460479488

Case Studies细胞转染 | LX2人肝星形细胞siRNA转染案例

📂 细胞转染 👁 7,592 🗓 2026-10-09

Source: Yeasen Biotech·https://www.yeasen.com/solutiondetail/2034526204631597056

Case Studies细胞转染 | MCF小鼠心脏成纤维细胞siRNA转染案例

📂 细胞转染 👁 16,911 🗓 2026-10-09

Source: Yeasen Biotech·https://www.yeasen.com/solutiondetail/2034527552623398912

Case Studies细胞转染 | RAW264.7小鼠单核巨噬细胞siRNA转染案例(苏州大学)

📂 细胞转染 👁 18,759 🗓 2026-10-09

Source: Yeasen Biotech·https://www.yeasen.com/solutiondetail/2034528410819670016

Case Studies细胞转染 | RAW264.7小鼠单核巨噬细胞siRNA转染案例(中国药科大学)

📂 细胞转染 👁 18,996 🗓 2026-10-09

Source: Yeasen Biotech·https://www.yeasen.com/solutiondetail/2034531938727104512

Case Studies细胞转染 | RBE大鼠胆管上皮细胞siRNA转染案例

📂 细胞转染 👁 15,826 🗓 2026-10-09

Source: Yeasen Biotech·https://www.yeasen.com/solutiondetail/2034533005763047424

Case Studies细胞转染 | STC1肠内分泌细胞siRNA转染案例

📂 细胞转染 👁 17,520 🗓 2026-10-09

Source: Yeasen Biotech·https://www.yeasen.com/solutiondetail/2034534417481211904

Case Studies细胞转染 | BMDM原代骨髓巨噬细胞siRNA转染案例(中国药科大学)

📂 细胞转染 👁 14,253 🗓 2026-10-09

Source: Yeasen Biotech·https://www.yeasen.com/solutiondetail/2034540025030438912

Case Studies细胞转染 | BMDM原代骨髓巨噬细胞siRNA转染案例(北京大学)

📂 细胞转染 👁 6,499 🗓 2026-10-09

Source: Yeasen Biotech·https://www.yeasen.com/solutiondetail/2034542304442740736

Case Studies细胞转染 | HUVEC人脐静脉内皮细胞siRNA转染案例

📂 细胞转染 👁 13,906 🗓 2026-10-09

Source: Yeasen Biotech·https://www.yeasen.com/solutiondetail/2034542964593446912

Case Studies细胞转染 | CEMT-4人类白血病T细胞siRNA转染案例

实验所用转染试剂试剂名称货号Hieff Trans®RNAiBoost Transfection Reagent转染试剂40807ES细胞培养与传代（T25）细胞计数：从培养箱取出细胞，细胞悬液吹打混匀，使用细胞计数仪检测细胞活率及密度。细胞传代（直接稀释法）：根据计数结果，将细胞按照5E5 cell/mL左右进行细胞传代。细胞传代（半换液法）：若待传代细胞

📂 细胞转染 👁 16,455 🗓 2026-10-09

Source: Yeasen Biotech·https://www.yeasen.com/solutiondetail/2034545751842131968

Case Studies细胞转染 | MCF-7人乳腺癌细胞siRNA转染案例

📂 细胞转染 👁 19,661 🗓 2026-10-09

Source: Yeasen Biotech·https://www.yeasen.com/solutiondetail/2034553302184923136

Case Studies细胞转染 | THP-1人单核细胞及诱导M0巨噬细胞siRNA转染案例

📂 细胞转染 👁 14,460 🗓 2026-10-09

Source: Yeasen Biotech·https://www.yeasen.com/solutiondetail/2034554210717847552

Case Studies细胞转染 | THP-1人单核细胞诱导M0巨噬细胞siRNA转染案例

📂 细胞转染 👁 17,029 🗓 2026-10-09

Source: Yeasen Biotech·https://www.yeasen.com/solutiondetail/2034555070989758464

Case Studies细胞转染 | BHK-21叙利亚仓鼠肾细胞DNA转染案例

实验所用转染试剂试剂名称货号Hieff Trans® LipoBooster 3000 Transfection Reagent Lipo3000转染试剂40801ES细胞培养与传代（T25）润洗：吸弃旧培养基，加入3-4 mL常温PBS轻晃润洗10-20秒后吸弃。消化：加入1 mL胰酶润湿瓶底，37°C孵育消化。吹落：待细胞间隙变大、形态变圆时，直接用胰酶

📂 细胞转染 👁 11,504 🗓 2026-10-09

Source: Yeasen Biotech·https://www.yeasen.com/solutiondetail/2035988010085158912

Case Studies细胞转染 | C2C12小鼠成肌细胞DNA转染案例

实验所用试剂清单试剂名称货号Hieff Trans® LipoBooster 3000 Transfection Reagent Lipo3000转染试剂40801ES细胞培养与传代（T25）润洗：吸弃旧培养基，加入3-4 mL常温PBS轻晃润洗10-20秒后吸弃。消化：加入1 mL胰酶润湿瓶底，37°C孵育消化。吹落：待细胞间隙变大、形态变圆时，直接用胰酶

📂 细胞转染 👁 9,179 🗓 2026-10-09

Source: Yeasen Biotech·https://www.yeasen.com/solutiondetail/1989147841495195648

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