Applications蛋白转运抑制剂--Brefeldin A布雷非德菌素A

Brefeldin A布雷非德菌素A，简称BFA，是一种从真菌中分离的内酯抗生素，是蛋白质运输的特定抑制剂。BFA可抑制coat proteins（如β-COP and ADP-ribosylation factor (ARF)）与高尔基体的结合并抑制GDP-GTP交换，破坏高尔基体的结构并抑制其功能，进而特异地阻断蛋白质从内质网(ER)向高尔基体(Golgi)的转运，也会导致膜蛋白分布受损。当作用于肿瘤细胞，BFA主要通过诱导分化和凋亡而发挥其细胞毒性作用。

图1.Brefeldin A化学结构式(CAS. NO: 20350-15-6)

BFA最初曾被用作抗病毒试剂，现在在细胞生物学主要用于研究蛋白转运,也常被用于抑制细胞因子等蛋白的分泌，并用于增强分泌蛋白的免疫染色检测。

图2.翌圣 50502ES03 Brefeldin A外观图

翌圣生物提供高纯度Brefeldin A布雷非德菌素A，纯度≥99%，为溶于DMSO的储存液，浓度5 mg/mL。Brefeldin A常见使用浓度范围为1-50μM。

图3.H1299细胞用Brefeldin A (5 μg/mL)处理，免疫荧光观察Hsp90a和CD63共定位情况，Brefeldin A不影响Hsp90α和CD63的膜转位。(PMID: 32640214)

图4.拟南芥幼苗用BFA (45 μM)处理0、30、60或90分钟，正常磷酸盐(NP；1.25 mM P i)或LP (0 mM P i)条件下，在BFA处理下或BFA冲洗后对BFA小体的观察和计数，结果显示：在NP和LP条件下，SNX1和SNX2a的内吞作用和胞吐作用水平相似；此外，与NP条件下相比，LP条件下PIN2的内吞和胞吐在pldζ2和snx1突变体或在PLD2抑制剂治疗下均未改。(PMID: 31745997)

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