Tag

#Transfection

All questions >

4ans

296views

The extracted plasmid has excessive endotoxin levels. Is there a separate remediation method to remove endotoxin?

Reagent Selection AAntibody2735 2026-07-12 #Endotoxin removal #Plasmid purification #Macrophages

4ans

247views

For primary cell transfection, which endotoxin-free plasmid extraction kit has the lowest residual?

Storage & Shipping AAndy2032 2026-04-01 #Plasmid extraction #Endotoxin removal #Primary cells

5ans

225views

Seeking advice on choosing AAV packaging transfection reagents: can they be used for both adherent 293 and suspension 293 cells?

Lab Techniques 邓邓燕杰1399 2026-08-28 #AAV packaging #Transfection reagent #293T

5ans

232views

Looking for recommendations for primary neuron transfection reagents; the cells are quite sensitive and I'm concerned about toxicity.

Storage & Shipping CCeller 2026-09-14 #Primary neurons #Transfection reagent #Low toxicity

5ans

218views

What transfection reagent yields high silencing efficiency for siRNA transfection in HepG2 cells?

Compliance WWang9305 2026-09-03 #siRNA #Transfection reagent #HepG2

5ans

169views

Which transfection reagent offers high efficiency and low cytotoxicity for transfecting plasmids into HEK293T cells?

Reagent Selection 黄黄宇569 2026-09-11 #Transfection reagent #HEK293T #Lentiviral packaging

4ans

158views

CRISPR library screening for tumor drug resistance genes: How to solve the issue of low lentiviral packaging titer?

Storage & Shipping 刘刘涛阳2427 2026-09-23 #CRISPR library #Lentiviral packaging #293T

4ans

242views

For CRISPR knockout in HeLa cells, please recommend Cas9 protein and sgRNA kits.

Storage & Shipping 朱朱娟6512 2026-06-02 #CRISPR-Cas9 #sgRNA #Gene knockout

5ans

193views

Transfection reagent for AAV packaging: How can the transfection efficiency of the three-plasmid system in HEK293T be improved?

Medical Devices KKevinBuffer4446 2026-09-16 #AAV packaging #HEK293T #Three-plasmid

5ans

280views

For suspension Jurkat cells, which is more suitable: electroporation or chemical transfection reagents? I'd like to hear from people who have used them.

Storage & Shipping LLisa9265 2026-08-26 #Jurkat #Suspension cells #Transfection reagent

5ans

240views

For transfecting primary neurons with siRNA, are there any recommended transfection reagents with low cytotoxicity?

Compliance LLilyBuffer2727 2026-09-15 #Primary neurons #siRNA #Cytotoxicity

5ans

224views

For lentiviral packaging in 293T cells, which transfection reagent offers high cost-effectiveness and stable lead times?

Lab Techniques 曹曹晨8654 2026-09-19 #Lentiviral packaging #293T #Transfection reagent

Community Rules

1SearchAvoid duplicates

2Be SpecificContext + tries + errors

3Answer WellGive steps and evidence

4AcceptBest answer first

Incentives

✓Points for accepted answers

✓Top answerers listed

✓Vendor support on board

✓Auto-linked products

Community Q&AQ&A CENTER

#Transfection

Community Rules

Incentives