How to confirm the specifications, stock, and delivery time for purchasing primary antibodies on the official website of Biyun Tian?

Spot and Arrival can't just look at product page titles, some are default delivery time templates. You can find the "spot/expected delivery" logo on the product page, and it is more reliable to send the product number to the official website online customer service or call 400 to ask: whether the Shanghai warehouse is in stock, what time can be paid before the same day, whether it is Fushun or ordinary courier. Shanghai same city spot usually arrives the next day, but cold shipping or dangerous goods may differ. It is recommended to note "Send SF Express, you need to confirm the order number" when placing the order, and ask the customer service to write the expected arrival in the note or chat record.

The prices on the official website are often similar to those of agents, with differences in terms of payment terms, shipping fees, and invoices. If a university research group registers a corporate/research account, there may be member discounts, full discounts, or point deductions, but they are not default. You can also ask the local agent to report the price including tax and shipping, and ask the official website if there are any laboratory purchase discounts or full gifts for the same item number. If the single amount is small, the official website shipping fee may not be cost-effective; If multiple items are added or combined at once, the official website may have an advantage. Prioritize confirming expiration date, batch number, and after-sales service when making purchases, rather than just comparing unit prices.

The selection of specifications mainly depends on the antibody dilution ratio and your experimental frequency. The general instruction manual recommends using approximately 5 μ l of 1:1000 and 5 ml incubation solution; 50 μ l specification can be used 8-10 times without any problem, while 100 μ l is more suitable for multi person sharing or subsequent IP/IF. It is recommended to purchase 50 μ l of new antibodies first to avoid leaving them unused due to inappropriate batch numbers or titers. After arrival, divide into 2-5 μ l/tube and store at -20 ℃ in the dark to avoid repeated freezing and thawing. The first time running WB, it is necessary to set up positive/negative controls, confirm the band specificity first, and then purchase in large quantities.