Can the FFPE tissue chip ensure that every point is cut out and fully organized?

There is no guarantee that every point will be cut out completely, but regular companies will have quality control. Many of the slices or disorganization you have encountered before are insufficient depth of embedding, excessive patching, or inadequate detachment prevention. When purchasing a lung cancer chip, it is recommended to directly ask the supplier to provide the HE quality control map or full scan from the same batch to confirm that each point is organized and the position is complete. Multi-label staining sample volume is small. It is recommended to have the supplier cut a sheet of HE and a homotype control first. You have seen it and then cut it in batches. The shedding rate can be agreed in the contract to organize the integrity rate, which does not reach the patch or refund.

The high slicing rate is related to the chip making process, slice thickness, and slide type. You are a multiple marker of lung cancer this time. It is recommended to choose a 2mm dot diameter instead of 1.5mm. The amount of tissue is larger, and it is not easy to remove after repeated elution. Positive charge anti-removal slides are explicitly required and baked. Once received, you can also bake your own 60°C flakes for 1-2 hours before dyeing. When selecting suppliers, ask: If you cut to the last few points, how to deal with after-sales? Manufacturers that dare to promise patches usually have more stable quality control.

Regarding the number of slices, lung cancer chips with a 2mm core diameter can theoretically cut 50-100 sheets, but the actual number of available sheets depends on the depth of the tissue column and the embedding situation. Multiple marks should be cycled multiple times, it is recommended not to put your wishes in the last few sheets, and estimate the dosage in advance: cut 20-30 sheets first, and leave 10-15 sheets for spare. The supplier is required to trim to the maximum surface and record the depth before slicing, so that the integrity of the front section is better. The HE quality control sheet must be in the same batch, preferably one at the beginning, one at the middle and one at the end, to determine whether the organization is continuously intact.

Multilabelling is most afraid of tissue loss and antigen loss. It is recommended that you prioritize chips with normal/cancer pairs for lung cancer and have the vendor provide the histological location of each point to avoid necrosis and fibrosis areas. Before buying, ask for the number of sheets that can be cut, don't just listen to the theoretical values, and ask them to provide the actual cut data or quality control records. After arrival, take one tablet to do HE to confirm that the points are complete; take another tablet to do a single label test to confirm the antigenicity. When batch dyeing, the slices are randomly divided into different batches to avoid system errors behind the slice position.