There are many brands of ELISA test kits, how to choose a new supplier for the laboratory?

In addition to R&D, Thermo, and Abcam, the inflammatory factor kits from BioLegend and BD OptEIA are also available for import. Some indicators have good stability and sensitivity, and their prices are usually milder than R&D. RayBiotech, Cayman, and Sino Biological also have coverage, but not all indicators are complete. Suggest asking the agent to provide COA for different batches of target indicators, including standard curve range, intra/inter batch CV, and sample type. Serum samples should pay attention to matrix effects, preferably confirmed by dilution linearity. Delivery time can be requested for domestic spot goods, which usually arrive within two weeks.

Your sample size is 48 holes at a time, and priority should be given to pre packaged boards and detachable strips to avoid wasting all at once. If the goods arrive within two weeks, we need to find a domestic agent or manufacturer with stock available for direct delivery. When inquiring, don't just look at the price, ask if it includes pre packaged boards, standard products, tested antibodies, etc HRP、 Substrate and termination solution, calculate the cost per pore. Sales can be requested to provide trial sets or small specifications for comparison, especially for new brands. At the same time as comparing prices, the stability of delivery time and batch number should be included in the procurement conditions to avoid the impact of subsequent batch fluctuations on longitudinal data.

Domestic Lilianke Biotechnology, Xinbosheng, Daktro Wei, Si Zheng Bai, Elabscience, Abclonal all have inflammatory factor lines. Intra-assay repeatability of domestic kits is generally possible, and inter-assay and low-concentration recovery requires self-verification. It is recommended to buy a 48-well small specification or apply for trial, test the same batch of serum sub-packaged samples in parallel with the old brand, and do high, medium, and low concentrations and spike recovery. Don't just look at the sensitivity of the instructions, the dilution linearity in the actual serum is more important. If the supplier can provide reference data for this laboratory sample type, it will be more certain.

Don't rush to compare prices when changing suppliers, use the same batch of samples for technical comparison first. Different brands of antibodies may have different sources of standards and absolute values, so we cannot only compare numerical values. Focus on whether the standard curve falls within the sample concentration range, whether the CV of the wells is controllable, and whether low concentration samples can be distinguished. It is recommended to centrifuge and separate serum samples before packaging and freezing to avoid repeated freeze-thaw cycles, otherwise batch differences may be mistakenly identified as reagent kit issues. The addition of known concentration standards for recovery rate can also be used to determine matrix interference.