Lab Techniques - BioReagent Hub

4ans

219views

For intraperitoneal injection of LPS in rats to establish an inflammatory model, which brand provides stable effects?

Lab Techniques SSample 2026-07-18 #Lipopolysaccharide #Animal models #Rat

4ans

195views

Does the dark color of the culture medium affect the readings when performing CCK-8 on primary cells extracted from animal tissues?

Lab Techniques 大大师达人 2026-07-28 #CCK-8 #Primary chondrocytes #Phenol red interference

4ans

165views

The animal facility requires separate recovery of ethidium bromide waste liquid. How does everyone handle this?

Lab Techniques KKiting6792 2026-08-15 #Ethidium bromide #Waste liquid recovery #Animal experiments

4ans

232views

When using Trizol to extract RNA from animal liver, the 260/230 ratio is only 1.2. Is this due to fat interference?

Lab Techniques 董董静娟 2026-07-02 #RNA extraction #Trizol #Animal liver

4ans

189views

When extracting protein from animal tissues, how long does PMSF remain effective after being added to the lysis buffer?

Lab Techniques PProtein2430 2026-04-18 #Phenylmethylsulfonyl fluoride #Protease inhibitor #Animal tissue

4ans

153views

Does DTT added to mouse embryo culture medium need to be filtered? What concentration is appropriate?

Lab Techniques LLeo3731 2026-03-01 #Embryo culture #DTT #Mouse

4ans

139views

For SDS purchased by the animal facility for use as electrophoresis buffer, is there a large purity difference between domestic and imported products?

Lab Techniques 曹曹文婷 2026-05-19 #SDS #Electrophoresis #Domestic reagents

4ans

157views

How many times should samples be diluted for the Coomassie Brilliant Blue method for goat milk whey protein detection?

Lab Techniques 郭郭鹏 2026-05-02 #Coomassie Brilliant Blue #Goat milk #Whey protein

4ans

253views

What is the lower limit of linearity for the BCA kit for low-concentration proteins in fish serum?

Lab Techniques CCellLine3177 2026-08-09 #BCA Assay Kit #Fish serum #Muscle tissue

4ans

196views

Immunofluorescence of fish tissue sections, permeabilization concentration of Triton X-100 and procurement issues.

Lab Techniques PPCRMax5123 2026-09-19 #Triton X-100 #Immunofluorescence #Zebrafish

4ans

218views

For cell culture, if you want to check for mycoplasma, how much does Giemsa staining differ in effectiveness from fluorescence methods?

Lab Techniques 肖肖阿宝6830 2026-04-09 #Cell culture #Mycoplasma testing #Giemsa staining

4ans

150views

Can Giemsa stain be used for mouse bone marrow smear counting?

Lab Techniques SSamReagent 2026-07-13 #Giemsa stain #Bone marrow smear #Micronucleus test

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