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5ans

228views

When using high-fidelity enzymes for site-directed mutagenesis, what should I do if the mutation efficiency remains low?

Lab Techniques NNina6324 2026-08-26 #High-fidelity enzyme #Site-directed mutagenesis #Plasmid PCR

4ans

193views

For monoclonal screening after CRISPR editing, is limiting dilution or flow cytometry sorting more reliable? How many cells are needed for T7E1?

Reagent Selection 梁梁伟 2026-06-20 #CRISPR #Monoclonal screening #T7E1

3ans

320views

What should be done if the starting DNA amount is insufficient for NGS library preparation in tumor mutation detection?

Lab Techniques 蒋蒋洋斌 2026-09-03 #NGS library preparation #Low input amount #Tumor mutation

3ans

158views

Tumor gene mutation detection kit tender: what is the price difference between domestic and imported products?

Reagent Selection 学学生小陈7751 2026-09-04 #Tumor gene mutation testing #Kit price comparison #EGFR

3ans

143views

For blood ctDNA tumor mutation detection, are there any kits with high extraction recovery rates that you would recommend?

Lab Techniques AAndy 2026-08-26 #ctDNA #Free DNA extraction #EGFR mutation

Recombinant Biotinyl...

3ans

244views

For mutation detection in FFPE tumor samples, how should one choose between NGS panel and ddPCR?

Storage & Shipping VViviExtract 2026-08-26 #Tumor mutation testing #FFPE #NGS

3ans

166views

For point mutations in a 3 kb fragment, is Q5 or KOD One high-fidelity polymerase better?

Lab Techniques EEmmaProtein8173 2026-08-28 #Tool enzymes #High-fidelity polymerase #Point mutation

3ans

205views

How effective is cfDNA targeted sequencing panel for detecting low-frequency mutations?

Lab Techniques LLisaScience 2026-09-14 #cfDNA #Targeted sequencing panel #Low-frequency mutation

4ans

201views

What level of purity is required for dNTPs used in cloning sequencing?

Lab Techniques SSample 2026-08-20 #dNTPs #HPLC purification #Cloning

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