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5ans

177views

Flow cytometry detection of CD molecules in human peripheral blood, direct-labeled primary antibody recommendations for clones and fluorophore combinations.

Compliance TTonyTube518 2026-09-03 #Flow cytometry #Directly labeled primary antibody #CD molecule

Recombinant Biotinyl...

5ans

190views

Which company offers the best cost-performance ratio for high-fidelity enzymes when amplifying 2 kb cDNA fragments?

Reagent Selection 唐唐博 2026-08-30 #High-fidelity enzyme #Molecular cloning #cDNA amplification

4ans

200views

For monoclonal screening after CRISPR editing, is limiting dilution or flow cytometry sorting more reliable? How many cells are needed for T7E1?

Reagent Selection 梁梁伟 2026-06-20 #CRISPR #Monoclonal screening #T7E1

4ans

194views

Seeking advice on antibody panel design for IL-17A and Foxp3 in Th17/Treg flow cytometry using mouse spleen single cells.

Compliance 陈陈小妹4696 2026-05-07 #Flow cytometry #Th17 #Treg

Recombinant Biotinyl...

4ans

249views

What validation is required for monoclonal antibodies used in ChIP experiments?

Reagent Selection OOscar4174 2026-05-16 #Monoclonal antibody #ChIP #Transcription factor

4ans

188views

How should the dosage of anti-human CD3 monoclonal antibody for flow cytometric detection of human T cell surface be titrated?

Reagent Selection AAntibodyZone1727 2026-05-03 #Monoclonal antibody #Flow cytometry #CD3

4ans

261views

The monoclonal antibody against a certain target for Western blot is out of stock. Could you recommend an alternative clone number?

Storage & Shipping 助助理老师 2026-04-25 #Monoclonal antibody #Western blot #Loading control antibody

Plant PDH E1αElisa K...

4ans

255views

Does different CD4-FITC antibody clone numbers affect mouse spleen cell subpopulations?

Storage & Shipping PPlate 2026-05-24 #Flow cytometry antibodies #CD4 #FITC

Recombinant Biotinyl...

4ans

206views

What level of purity is required for dNTPs used in cloning sequencing?

Lab Techniques SSample 2026-08-20 #dNTPs #HPLC purification #Cloning

4ans

254views

Is there a significant difference in cutting efficiency between domestically produced restriction endonucleases and imported ones? Due to limited budget, I want to switch to domestic products

Medical Devices PPlate 2026-09-11 #Restriction endonuclease #Domestic reagents #Cost-effectiveness

4ans

171views

Can we use regular Taq for cloning construction? Do we still need high fidelity enzymes?

Reagent Selection SSamSample482 2026-04-16 #Taq DNA polymerase #Cloning construction #Fidelity

0ans

251views

How to select batches of anti-PD-1 antibodies for mouse models with immune side effects?

Supplier & Sourcing 冯冯刚9923 2026-08-05 #Monoclonal antibody #PD-1 #Immune side effects

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