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Community Q&A

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Ask and answer experiment and selection questions. Every question links to related products.

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#Primer

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5ans
213views

Will adding too much reverse transcriptase inhibit downstream qPCR? How many units are generally appropriate to add to a 20 µL system?

Storage & Shipping NNinaAssay36 2026-09-13 #Reverse transcriptase #RT-qPCR #System optimization
3ans
221views

Will repeated freeze-thaw cycles after dissolving primer and probe dry powder affect the experimental results?

Lab Techniques 细细胞君 2026-08-26 #Primer storage #Probe stability #Freeze-thaw
3ans
261views

In multiplex qPCR, will the fluorescent groups of different probes interfere with each other?

Lab Techniques MMediaer111 2026-09-06 #Multiplex qPCR #Fluorescent labeling #Primer and probe
3ans
205views

For bulk customized primers and probes, which company offers better lead times and pricing?

Supplier & Sourcing LLabBase2006 2026-09-18 #Primer synthesis #Probe customization #Price comparison
3ans
150views

How should parameters be adjusted for nonspecific amplification of qPCR primers and probes?

Reagent Selection 董董耗材 2026-09-13 #qPCR #Primer design #Probe optimization
4ans
188views

Will buying enzymes and primers separately from the reverse transcription kit reduce their universality?

Reagent Selection LLily1357 2026-06-05 #Reverse transcriptase #Two-step method #Primer

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