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#Methodology

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4ans

189views

When extracting protein from animal tissues, how long does PMSF remain effective after being added to the lysis buffer?

Lab Techniques PProtein2430 2026-04-18 #Phenylmethylsulfonyl fluoride #Protease inhibitor #Animal tissue

4ans

275views

For trypan blue used with an automated cell counter, how much difference from manual counting results is considered normal?

Compliance 董董强4334 2026-09-15 #Trypan blue #Automated cell counter #Fibroblast

4ans

178views

Stain PBMCs with trypan blue; there are many blue dots in the background—are they debris or dye precipitate?

Storage & Shipping WWendyExtract4190 2026-04-30 #Trypan blue #PBMC #Cell counting

5ans

200views

Does the direct switch of CHO cells from serum-containing to serum-free medium require an adaptation step?

Medical Devices LLisaLab 2026-09-01 #Serum-free medium #CHO cells #Domestication

4ans

183views

After purification of His-tagged recombinant protein using a nickel column, the bands are impure. How can I optimize the conditions?

Compliance AAndy2058 2026-08-07 #Recombinant protein #His tag #Nickel column purification

Recombinant Biotinyl...

0ans

178views

For pathogen nucleic acid testing, if you want to switch from qPCR to isothermal amplification, how should reagents and instruments be selected?

Reagent Selection KKitMax9418 2026-08-28 #Isothermal amplification #LAMP #RPA

3ans

235views

For mutation detection in FFPE tumor samples, how should one choose between NGS panel and ddPCR?

Storage & Shipping VViviExtract 2026-08-26 #Tumor mutation testing #FFPE #NGS

3ans

261views

In multiplex qPCR, will the fluorescent groups of different probes interfere with each other?

Lab Techniques MMediaer111 2026-09-06 #Multiplex qPCR #Fluorescent labeling #Primer and probe

3ans

149views

Can microfluidic chips be used for plasma exosome isolation?

Medical Devices CCellTech4667 2026-09-12 #Exosomes #Microfluidic chip #Separation and purification

3ans

315views

Can phosphorylated protein microarrays use RIPA to lyse cells? Will the background be very high?

Storage & Shipping BBioPro7367 2026-09-23 #Phosphorylated protein microarray #Sample preparation #RIPA

4ans

180views

How should antigen retrieval conditions be optimized for multiplex immunofluorescence on paraffin-embedded tissue microarrays?

Lab Techniques MMediaing931 2026-06-30 #Paraffin-embedded tissue microarray #Multiplex immunofluorescence #Antigen retrieval

4ans

202views

For multiplex immunohistochemistry using pathology diagnostic chips on FFPE samples, the background is high. How can it be optimized?

Medical Devices VViviResearch7585 2026-04-08 #FFPE #Multiplex immunohistochemistry #Pathology diagnostic chip

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