Q&A Community - BioReagent Hub

4ans

252views

Looking for recommendations for IL-6 recombinant protein for use in ELISA standard curves. Is the activity consistent across batches?

Lab Techniques LLisaReagent5658 2026-09-02 #Recombinant protein #ELISA #IL-6

ELISA试剂盒

Plant PDH E1αElisa K...

4ans

191views

After purification of His-tagged recombinant protein using a nickel column, the bands are impure. How can I optimize the conditions?

Compliance AAndy2058 2026-08-07 #Recombinant protein #His tag #Nickel column purification

Recombinant Biotinyl...

5ans

187views

Using the same kit to extract bacterial plasmids and genomic DNA, what should I do if the 260/230 ratio is always low?

Reagent Selection 曹曹杰倩 2026-09-18 #Plasmid extraction #Genomic DNA #260/230

5ans

234views

Can the viral RNA extraction kit be used for both nasal swab and bronchoalveolar lavage fluid samples?

Lab Techniques RRex1335 2026-09-12 #Viral RNA #Column method #Respiratory tract samples

5ans

245views

For plant leaf samples rich in polysaccharides and polyphenols, which DNA extraction kit has a fast lysis speed?

Lab Techniques PProteinTech2875 2026-09-21 #Plant DNA #Polysaccharides and polyphenols #High-throughput

5ans

231views

For extracting plasma cell-free DNA using the magnetic bead method, are there any kits recommended that can remove heparin inhibition?

Reagent Selection LLab9067 2026-09-15 #Free nucleic acid #Magnetic bead method #Plasma

5ans

193views

Transfection reagent for AAV packaging: How can the transfection efficiency of the three-plasmid system in HEK293T be improved?

Medical Devices KKevinBuffer4446 2026-09-16 #AAV packaging #HEK293T #Three-plasmid

5ans

282views

For suspension Jurkat cells, which is more suitable: electroporation or chemical transfection reagents? I'd like to hear from people who have used them.

Storage & Shipping LLisa9265 2026-08-26 #Jurkat #Suspension cells #Transfection reagent

5ans

242views

For transfecting primary neurons with siRNA, are there any recommended transfection reagents with low cytotoxicity?

Compliance LLilyBuffer2727 2026-09-15 #Primary neurons #siRNA #Cytotoxicity

5ans

227views

For lentiviral packaging in 293T cells, which transfection reagent offers high cost-effectiveness and stable lead times?

Lab Techniques 曹曹晨8654 2026-09-19 #Lentiviral packaging #293T #Transfection reagent

5ans

188views

When ordering factors such as SCF and TPO for stem cell culture, how do you convert the units, and how do you confirm the shelf life?

Medical Devices RRex 2026-09-05 #Stem cell culture #Cytokine #Unit conversion

5ans

189views

The mouse serum sample volume is limited, and I want to measure multiple cytokines in one run. Should I choose CBA or Luminex?

Medical Devices 研研发达人2657 2026-09-08 #Multiplex assay #Cytokine #Flow cytometry

Plant PDH E1αElisa K...

HMG-COA Elisa Kit

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