Q&A Community - BioReagent Hub

4ans

212views

For primary cells extracted from porcine tissue, should the penicillin-streptomycin dosage be doubled?

Supplier & Sourcing BBufferLine 2026-08-19 #Penicillin-Streptomycin #Primary extraction #Porcine tissue

4ans

265views

After adding antibiotics to mouse splenocytes, the viability decreased significantly. Is this an antibiotic issue?

Reagent Selection MMarkAntibody6343 2026-04-27 #Penicillin-Streptomycin #Mouse splenocytes #Lymphocytes

4ans

304views

When purchasing large quantities of dual antibiotics for animal facilities, how should one choose between domestic and imported options considering lead time and price?

Compliance 冯冯研发 2026-03-03 #Penicillin-Streptomycin #Procurement price comparison #Lead time

4ans

195views

Will adding penicillin-streptomycin to primary rat fibroblasts affect their growth?

Storage & Shipping RReagenting8982 2026-09-07 #Penicillin-Streptomycin #Primary cell culture #Cytotoxicity

4ans

235views

How much does DMSO residue in monoclonal antibody purification samples affect mouse tail vein injection?

Supplier & Sourcing BBuffering7757 2026-06-29 #Dimethyl sulfoxide #Monoclonal antibody purification #Mouse injection

4ans

241views

Is there a big difference between domestic and imported DMSO used as a solvent for drug administration in mice in animal experiments?

Lab Techniques 彭彭检测 2026-04-09 #Dimethyl sulfoxide #Domestic/Imported #Mouse gavage

4ans

276views

For animal experiments, is a DMSO concentration of 10% or 5% more appropriate for rat liver tissue cryopreservation solution?

Reagent Selection BBio 2026-04-24 #Dimethyl sulfoxide #Tissue cryopreservation #Rat

4ans

260views

What is the appropriate safe concentration of DMSO as a solvent for intraperitoneal injection in animal experiments using mice?

Lab Techniques 郑郑杰7662 2026-07-31 #Dimethyl sulfoxide #Animal experiments #Intraperitoneal injection

4ans

300views

For trypan blue used with an automated cell counter, how much difference from manual counting results is considered normal?

Compliance 董董强4334 2026-09-15 #Trypan blue #Automated cell counter #Fibroblast

4ans

199views

Stain PBMCs with trypan blue; there are many blue dots in the background—are they debris or dye precipitate?

Storage & Shipping WWendyExtract4190 2026-04-30 #Trypan blue #PBMC #Cell counting

4ans

208views

Which packaging of trypan blue is suitable to buy? There is a big price difference between 10 mL and 100 mL.

Medical Devices AAntibody 2026-08-28 #Trypan blue #Cell counting #Reagents and consumables

4ans

200views

When performing primary hepatocyte counting, why is the viability always low after trypan blue staining?

Medical Devices 唐唐强阳 2026-09-21 #Trypan blue #Primary cells #Cell counting

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