Tag

#Reverse transcriptase

All questions >

5ans

213views

Will adding too much reverse transcriptase inhibit downstream qPCR? How many units are generally appropriate to add to a 20 µL system?

Storage & Shipping NNinaAssay36 2026-09-13 #Reverse transcriptase #RT-qPCR #System optimization

5ans

173views

When performing RNA reverse transcription from plant leaves, inhibition often occurs. Which reverse transcriptase has strong resistance to polysaccharides and polyphenols?

Reagent Selection BBufferLab6335 2026-09-16 #Reverse transcriptase #Plant RNA #Polysaccharides and polyphenols

5ans

228views

Procurement inquiry: How are the lead times for imported reverse transcriptase kits recently? Are there any reliable domestic alternatives?

Lab Techniques 杨杨小妹6241 2026-09-22 #Reverse transcriptase #Lead time #Domestic substitution

5ans

217views

For reverse transcription of low-copy genes, is ordinary M-MLV or thermostable reverse transcriptase more suitable?

Storage & Shipping 唐唐敏 2026-08-29 #Reverse transcriptase #Low-copy gene #cDNA synthesis

3ans

263views

For qPCR with low-input RNA, which reverse transcriptase brand is more reliable?

Reagent Selection 彭彭静斌8274 2026-08-28 #Tool enzymes #Reverse transcriptase #Low-input RNA

Recombinant Biotinyl...

4ans

188views

Will buying enzymes and primers separately from the reverse transcription kit reduce their universality?

Reagent Selection LLily1357 2026-06-05 #Reverse transcriptase #Two-step method #Primer

4ans

246views

Can reverse transcriptase be sold as a whole in bulk packaging? How is the validity period calculated

Reagent Selection 研研发老师5514 2026-07-12 #Reverse transcriptase #Bulk packaging #Aliquoting

4ans

240views

Does the presence or absence of RNase H activity have a significant impact on subsequent qPCR?

Compliance 老老王同学 2026-08-08 #Reverse transcriptase #RNase H #AMV

4ans

325views

How to choose reverse transcriptase for RT qPCR of low copy genes?

Supplier & Sourcing WWangResearch7772 2026-04-14 #Reverse transcriptase #RT-qPCR #Low copy

4ans

198views

Is the imported M-MLV reverse transcriptase currently out of stock? Seeking alternatives

Storage & Shipping 冯冯飞飞 2026-05-31 #Reverse transcriptase #M-MLV #Lead time

Community Rules

1SearchAvoid duplicates

2Be SpecificContext + tries + errors

3Answer WellGive steps and evidence

4AcceptBest answer first

Incentives

✓Points for accepted answers

✓Top answerers listed

✓Vendor support on board

✓Auto-linked products

Community Q&AQ&A CENTER

#Reverse transcriptase

Community Rules

Incentives