Q&A Community - BioReagent Hub

4ans

308views

Does intragastric administration of LPS to mice cause intestinal inflammation? Is it necessary to prepare it with ultrapure endotoxin-free water?

Medical Devices 余余文3761 2026-07-04 #Lipopolysaccharide #Gavage #Mouse model

4ans

167views

For poultry immune experiments, which source of LPS is suitable? O111:B4 or O55:B5?

Storage & Shipping WWang2345 2026-09-06 #Lipopolysaccharide #Poultry experiment #Serotype

4ans

164views

After preparing the LPS solution, store it at -20°C. Will repeated freeze-thaw cycles affect the effect when administered to mice?

Storage & Shipping LLeo6613 2026-07-01 #Lipopolysaccharide #Mouse experiment #Storage conditions

4ans

233views

For intraperitoneal injection of LPS in rats to establish an inflammatory model, which brand provides stable effects?

Lab Techniques SSample 2026-07-18 #Lipopolysaccharide #Animal models #Rat

4ans

270views

Can the FITC channel be used with this apoptosis kit for testing chicken peripheral blood lymphocytes?

Reagent Selection 试试剂博士8080 2026-08-10 #Avian lymphocytes #Species suitability #Annexin V

4ans

143views

After grinding tumor tissue blocks, single cells stained with Annexin V/PI show many fragments. How should gating be performed?

Reagent Selection RResearch 2026-04-02 #Tumor tissue #Single-cell suspension #Flow cytometry gating

4ans

185views

For apoptosis experiments using primary rat hepatocytes, will trypsin digestion affect Annexin V staining?

Medical Devices 潘潘宇琳4195 2026-09-08 #Primary hepatocytes #Trypsin digestion #Annexin V

4ans

245views

Does the apoptosis kit require separate compensation adjustment when used with mouse splenocytes?

Storage & Shipping AAntibodyer2477 2026-06-29 #Mouse splenocytes #Flow cytometry compensation #Annexin V-FITC

4ans

210views

Does the dark color of the culture medium affect the readings when performing CCK-8 on primary cells extracted from animal tissues?

Lab Techniques 大大师达人 2026-07-28 #CCK-8 #Primary chondrocytes #Phenol red interference

4ans

251views

Can the CCK-8 kit still be used after repeated freeze-thaw cycles? Should it be aliquoted or stored as a whole bottle at 4°C?

Medical Devices LLisa 2026-07-01 #CCK-8 #Storage conditions #Repeated freeze-thaw

4ans

233views

For those raising animals, let me ask: can CCK-8 be used to measure suspension cells? Which is more accurate compared with MTT?

Supplier & Sourcing 周周琳斌3565 2026-04-07 #CCK-8 #Suspension cells #MTT comparison

4ans

266views

For measuring primary hepatocyte viability with a CCK-8 kit, what size package is sufficient?

Storage & Shipping LLily 2026-03-29 #CCK-8 #Primary hepatocytes #Cytotoxicity

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