Permeabilization Wash Buffer 破膜剂
勾选需要的规格可一次提交;也可以逐个询价。货号会一并带入询价单。
Q:做核内因子的流式分析,这个破膜剂能破核膜吗?
A:不能,皂素是相对理想的细胞破膜剂,但无法破核膜,若需破核膜还是要用 Triton-X 和Tween-20。
Q:破膜剂能不能先染膜抗体,然后破膜后再染细胞里面的,还是说需要破膜之后一起染?
A:先固定,再破膜,再染色,需要破膜之后一起染。
Q: 这个破膜剂破膜是可逆的吗?用于免疫荧光中,使用该破膜剂后封闭液、抗体稀释液及洗涤液是否都可以使用这款破膜剂?
A: 是可逆的,该破膜剂为皂素。封闭液推荐使用该破膜剂配置1%的BSA,同样可以使用该破膜剂用于抗体稀释以及洗涤液。
已发表文献
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[6] Shi S, Wu X, Wang X, et al. Differentiation of Bone Marrow Mesenchymal Stem Cells to Cardiomyocyte-Like Cells Is Regulated by the Combined Low Dose Treatment of Transforming Growth Factor-β1 and 5-Azacytidine. Stem Cells Int. 2016;2016:3816256. doi:10.1155/2016/3816256(IF:2.813)
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