YSFluor® 488 EdU Fluorescence Imaging & Flow Cytometry Assay kit YSFluor®488 Click-iT EdU通用款细胞增殖检测试剂盒（绿色荧光）

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荧光显微镜流式细胞仪细胞健康评估遗传毒性检测抗肿瘤药物筛选

Product no.40275ES Sizes2 options Documents1 files SourceYeasen · Official

Cat. No. 40275ES60

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40275ES60 100 T/10 T ¥1,508.00促~~¥1,583.00~~ 现货 Enquire

40275ES76 500 T/50 T ¥3,028.00促~~¥3,183.00~~ 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No40275ES

Specification试剂盒中EdU试剂含有炔烃，而Yefluor 488 Azide染料试剂含有叠氮化合物。点击法的EdU标记增殖快速有效，易于使用。只需少量的叠氮化染料即可有效地标记出整合的EdU。标准化的戊二醛固定和去污剂促渗可以使检测试剂进入细胞，而Br

Origin翌圣生物科技(上海)股份有限公司

技术平台 荧光显微镜 流式细胞仪

应用方法 细胞健康评估评估细胞健康程度 遗传毒性检测检测遗传毒性 抗肿瘤药物筛选评估抗肿瘤药物效果

检测方法 EdU标记法

生物特性参数 检测靶标EdU（5-溴-2-脱氧尿嘧啶） 细胞周期标记Hoechst 33342 DNA合成期标记EdU可整合入DNA双链

样本类型细胞

技术参数浓度10 mM EdU 储存条件-25~-15℃避光储存；开封后组分A和B需-25~-15℃避光，其余组分2-8℃ 有效期1年 荧光标记Yefluor 488（绿色荧光） 检测原理点击反应（铜催化的叠氮化合物与炔烃共价反应）

实验类型 细胞增殖检测通过EdU掺入DNA合成期检测细胞增殖能力 细胞周期分析结合Hoechst 33342染料分析细胞周期分布

Documents & Citations

细胞功能检测和探针整体方案 Open document

Published citations

[1] Lu X, Ma L, Yin X, et al. The impact of tobacco exposure on tumor microenvironment and prognosis in lung adenocarcinoma by integrative analysis of multi-omics data. Int Immunopharmacol. 2021;101(Pt B):108253. doi:10.1016/j.intimp.2021.108253(IF:4.932)

[2] Wang Q, Liang D, Shen P, Yu Y, Yan Y, You W. Hsa_circ_0092276 promotes doxorubicin resistance in breast cancer cells by regulating autophagy via miR-348/ATG7 axis. Transl Oncol. 2021;14(8):101045. doi:10.1016/j.tranon.2021.101045(IF:4.243)

[3] Wang J, Zeng Z, Lei S, et al. Sinapine Thiocyanate Inhibits the Proliferation and Mobility of Pancreatic Cancer Cells by Up-Regulating GADD45A. J Cancer. 2022;13(4):1229-1240. Published 2022 Jan 24. doi:10.7150/jca.65212(IF:4.207)

[4] Guan X, Guan X, Wang Y, et al. Circ_0003340 downregulation mitigates esophageal squamous cell carcinoma progression by targeting miR-940/PRKAA1 axis. Thorac Cancer. 2022;13(8):1164-1175. doi:10.1111/1759-7714.14377(IF:3.500)