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产品资料 PD-40202ES
阿尔玛蓝试剂盒 阿尔玛蓝染色试剂 阿尔玛蓝试剂盒 阿尔玛蓝染色试剂
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阿尔玛蓝试剂盒 阿尔玛蓝染色试剂

Alamar Blue 阿尔玛蓝

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分光光度计荧光光度计细胞生物学药物筛选微生物学
产品编号40202ES 可选规格4 种 配套文档1 份 来源翌圣生物 · 官网
产品编号 40202ES60
现货 ¥98.00
数量 1
立即询价
询价 / 批量采购 下载规格书 加入比价

规格与询价

勾选需要的规格可一次提交;也可以逐个询价。货号会一并带入询价单。

货号 规格 价格 状态
40202ES60 100 T(1 mL) ¥98.00 现货 询价
40202ES76 500 T(5 mL) ¥308.00促¥320.00 现货 询价
40202ES80 1000 T(10 mL) ¥578.00促¥600.00 现货 询价
40202ES92 10000 T(100 mL) ¥2,058.00促¥2,162.00 现货 询价
提交询价 价格为协议价,提交后由业务确认;大包装可申请试用装。

产品概述

技术参数

品牌翌圣生物 Yeasen
货号 / 型号40202ES
规格本品可广泛地用于细胞增殖代谢,药物的细胞毒作用的体外测定以及病原微生物的的快速检测与鉴定。 与台盼兰、TTC、MTT、MTS等分析法相比,Alamar Blue具有更多的优势。Alamar Blue采用单一试剂,可以连续、快速地检测细胞的增
产地翌圣生物科技(上海)股份有限公司
技术平台 分光光度计 荧光光度计
应用方法 细胞生物学细胞活性与增殖分析 药物筛选药物细胞毒作用测定 微生物学病原微生物快速检测与鉴定
适用物种种属 动物 真菌 细菌
检测方法 比色法 荧光法
生物特性参数 应用细胞增殖代谢、药物细胞毒作用测定、病原微生物快速检测与鉴定
样本类型 细胞
技术参数 储存条件4ºC避光保存,至少一年有效 运输条件冰袋(wet ice)运输 激发光波长530-560 nm 发射光波长590 nm 检测原理氧化还原指示剂,根据代谢活性产生吸光度变化和荧光信号
实验类型 细胞活力检测检测细胞代谢活性 细胞增殖检测定量分析细胞增殖 细胞毒性检测体外细胞毒性研究

常见问题

Q:还原率的计算其中一步是还原型阳性对照荧光值减去阴性对照的荧光值,减去后成负值?

A:阳性对照的值比实验组高,需要重新制备。阳性对照是 100%还原的,高温使得阿尔玛蓝变成还原态的。

Q:不能用PBS配制溶液,因为100%还原型Alamar Blue在PBS中不稳定,具体的原理是什么?

A:磷酸根会与Alamar Blue反应

Q:Alamar Blue 阿尔玛蓝,使用是需要高压灭菌是吧?

A: 阿尔玛蓝本身是无菌的。只有制备还原型的时候需要灭菌。不能对浓缩的Alamar Blue高压灭菌。

文档与文献

已发表文献

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[10] Chen Z, Zhang Z, Chen M, Xie S, Wang T, Li X. Synergistic antitumor efficacy of hybrid micelles with mitochondrial targeting and stimuli-responsive drug release behavior. J Mater Chem B. 2019;7(9):1415-1426. doi:10.1039/c8tb02843e(IF:5.047)

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[13] Lu J, Hu X, Yuan T, et al. 3D-Printed Poly (P-Dioxanone) Stent for Endovascular Application: In Vitro Evaluations. Polymers (Basel). 2022;14(9):1755. Published 2022 Apr 26. doi:10.3390/polym14091755(IF:4.329)

[14] Guo H, Wang Z, Du Q, Li P, Wang Z, Wang A. Stimulated phase-shift acoustic nanodroplets enhance vancomycin efficacy against methicillin-resistant Staphylococcus aureus biofilms. Int J Nanomedicine. 2017;12:4679-4690. Published 2017 Jun 30. doi:10.2147/IJN.S134525(IF:4.300)

[15] Chen Z, Chen C, Zhou T, et al. A high-throughput drug combination screen identifies an anti-glioma synergism between TH588 and PI3K inhibitors. Cancer Cell Int. 2020;20:337. Published 2020 Jul 23. doi:10.1186/s12935-020-01427-0(IF:4.175)

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