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Product data sheet PD-96481ES
Recombinant Biotinylated SARS-COV-2 Spike RBD Protein,His-Avi Tag Recombinant Biotinylated SARS-COV-2 Spike RBD Protein,His-Avi Tag
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Recombinant Biotinylated SARS-COV-2 Spike RBD Protein,His-Avi Tag

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HEK293细胞表达系统新冠相关研究
Product no.96481ES Sizes3 options SourceYeasen · Official
Cat. No. 96481ES25
现货 $1,975.00
Qty 1
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Cat.No Spec Price Status
96481ES25 25 μg $1,975.00 现货 Enquire
96481ES60 100 μg $7,885.00 现货 Enquire
96481ES76 500 μg $26,255.00 现货 Enquire
Submit enquiry Price is on application; bulk sizes can be sampled on request.

Overview

Specifications

Brand翌圣生物 Yeasen
Cat.No96481ES
Specification高活性、高纯度、低内毒素、高批间一致性
Origin翌圣生物科技(上海)股份有限公司
技术平台 HEK293细胞表达系统
应用方法 新冠相关研究研究SARS-CoV-2 Spike蛋白RBD与ACE2受体的相互作用
适用物种种属 SARS-CoV-2
检测方法 SDS-PAGE SEC-HPLC LAL法 ELISA Bis-Tris PAGE
生物特性参数 蛋白类型重组蛋白(生物素化SARS-CoV-2 Spike RBD蛋白) 种属来源SARS-CoV-2 生物活性描述Biotinylated SARS-CoV-2 Spike RBD His Tag与Human ACE2 hFc Tag结合的EC50为7.7 ng/ml(ELISA测定) 应用研究SARS-CoV-2 Spike蛋白RBD与ACE2受体的结合机制
技术参数 纯度>95% as determined by SDS-PAGE and SEC-HPLC 内毒素水平Less than 1 EU per μg by the LAL method 分子量Predicted MW 27 kDa; migrates to 36-40 kDa due to glycosylation by Bis-Tris PAGE 制剂形式Lyophilized from 0.22μm filtered solution in PBS (pH 7.4); normally 8% trehalose added as protectant before lyophilization 表达系统HEK293 标签C-His-Avi 表达序列Arg319-Asn532 AccessionQHD43416.1 外观Lyophilized Powder 生物素标记Biotinylated (Avi tag) 复溶方法用蒸馏水溶解冻干的蛋白。25μg或100μg建议复溶到0.5mg/mL;500μg或1mg建议以1mg/mL浓度复溶。复溶过程中不要剧烈涡旋或摇晃,分装后储存于-80℃,避免反复冻融。 储存条件-25~-15℃保存,收到货之日起有效期1年;复溶后-85~-65℃保存3个月;避免反复冻融。 活性ELISA: EC50 of 7.7 ng/ml for binding to Human ACE2 hFc Tag 产品特性高活性、高纯度、低内毒素、高批间一致性
实验类型 ELISA测定RBD与ACE2的结合活性 SDS-PAGE测定蛋白纯度 SEC-HPLC测定蛋白纯度 LAL法测定内毒素水平 Bis-Tris PAGE测定蛋白分子量

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