Recombinant SARS-CoV-2 Spike RBD (Omicron BA.2.75.2) Protein,His Tag

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HEK293细胞表达系统新冠相关研究

Product no.96462ES Sizes3 options SourceYeasen · Official

Cat. No. 96462ES25

现货 $1,295.00

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96462ES25 25 μg $1,295.00 现货 Enquire

96462ES60 100 μg $5,185.00 现货 Enquire

96462ES76 500 μg $17,255.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No96462ES

Specification高活性、高纯度、低内毒素、高批间一致性

Origin翌圣生物科技(上海)股份有限公司

技术平台 HEK293细胞表达系统

应用方法 新冠相关研究用于研究SARS-CoV-2 Spike RBD与ACE2的相互作用

适用物种种属 SARS-CoV-2

检测方法 SDS-PAGE SEC-HPLC LAL法 ELISA

生物特性参数 蛋白类型重组蛋白 种属来源SARS-CoV-2 (Virus) 生物活性描述ELISA Data: Immobilized SARS-CoV-2 Spike RBD (Omicron BA.2.75.2) His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human ACE2 hFc Tag with the EC50 of 8.9ng/ml determined by ELISA. 应用新冠相关研究，用于研究Spike RBD与ACE2的相互作用 Accession号QHD43416.1 表达序列Arg319-Phe541(G339H R346T S371F S373P S375F T376A D405N R408S K417N N440K G446S N460K S477N T478K E484A F486S Q498R N501Y Y505H)

技术参数纯度> 95% as determined by SDS-PAGE and SEC-HPLC 内毒素水平Less than 1 EU per μg by the LAL method. 分子量Predicted MW 26.28 kDa; migrates to 35-43 kDa by Bis-Tris PAGE 制剂形式Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. 储存条件-25~-15℃保存，有效期1年；复溶后-85~-65℃保存，3个月；避免反复冻融。 表达系统HEK293 标签C-His 外观Lyophilized Powder 复溶方法用蒸馏水溶解冻干的蛋白。如果规格为25μg或者100μg，建议复溶到0.5mg/mL的浓度。对于500μg或1mg的冻干粉，建议以1mg/mL的浓度复溶。复溶过程中请不要剧烈涡旋或者剧烈摇晃。将复溶后的蛋白溶液按照需求进行分装，储存于-80℃，避免反复冻融。