Recombinant Mouse Coagulation factor III/Tissue Factor Protein,His Tag

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HEK293表达系统凝血研究抗体药物靶点研究

Product no.96335ES Sizes3 options SourceYeasen · Official

Cat. No. 96335ES25

现货 ¥845.00

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96335ES25 25 μg ¥845.00 现货 Enquire

96335ES60 100 μg ¥3,385.00 现货 Enquire

96335ES76 500 μg ¥11,255.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No96335ES

Specification高活性、高纯度、低内毒素、高批间一致性

Origin翌圣生物科技(上海)股份有限公司

技术平台 HEK293表达系统

应用方法 凝血研究组织因子是外源性凝血途径的关键启动因子，用于研究凝血机制 抗体药物靶点研究Tissue factor/CD142为靶点蛋白，可用于抗体药物筛选与开发

适用物种种属小鼠

检测方法 SDS-PAGE SEC-HPLC LAL法 Bis-Tris PAGE 荧光肽底物法

生物特性参数 蛋白类型组织因子/凝血因子III (Tissue factor/Coagulation Factor III) 种属来源Mouse 生物活性描述Measured by its ability to activate Coagulation Factor VII in cleaving a fluorogenic peptide substrate Boc-VPR-AMC. The AC50 is <5 µg/mL. 应用组织因子是外源性凝血途径的主要细胞启动因子，适用于凝血机制研究和以Tissue factor/CD142为靶点的抗体药物研发

样本类型 重组蛋白

技术参数纯度> 95% as determined by SDS-PAGE and SEC-HPLC 内毒素水平Less than 1 EU per μg by the LAL method 分子量预测分子量27 kDa；糖基化后经Bis-Tris PAGE检测迁移至42-55 kDa 制剂形式Lyophilized from 0.22 μm filtered solution in 50mM Tris 150mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization. 活性Bioactivity Data: Measured by its ability to activate Coagulation Factor VII in cleaving a fluorogenic peptide substrate Boc-VPR-AMC. The AC50 is <5 µg/mL. 储存条件-25~-15℃保存，收到货之日起，有效期1年；复溶后，-85~-65℃保存，3个月有效期；避免反复冻融 表达系统HEK293 标签C-His AccessionP20352 表达序列Ala29-Glu251 复溶方法用蒸馏水溶解冻干蛋白。25μg或100μg建议复溶到0.5mg/mL；500μg或1mg建议以1mg/mL复溶。复溶过程中不要剧烈涡旋或摇晃。分装后储存于-80℃，避免反复冻融。外观Lyophilized Powder pH7.5

实验类型 活性检测测定蛋白激活Coagulation Factor VII切割荧光底物Boc-VPR-AMC的能力，AC50 <5 µg/mL 纯度检测通过SDS-PAGE和SEC-HPLC验证蛋白纯度 >95% 内毒素检测通过LAL法检测内毒素水平 <1 EU/μg 分子量分析通过Bis-Tris PAGE分析糖基化后蛋白分子量迁移至42-55 kDa