Recombinant FITC-Labeled Human CD5 Protein,His Tag

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HEK293表达系统CAR-T

Product no.96222ES Sizes3 options SourceYeasen · Official

Cat. No. 96222ES25

现货 ¥3,715.00

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Cat.No Spec Price Status

96222ES25 25 μg ¥3,715.00 现货 Enquire

96222ES60 100 μg ¥13,505.00 现货 Enquire

96222ES76 500 μg ¥30,005.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No96222ES

Specification高活性、高纯度、低内毒素、高批间一致性

Origin翌圣生物科技(上海)股份有限公司

技术平台 HEK293表达系统

应用方法 CAR-T靶点蛋白

适用物种种属人

检测方法 SDS-聚丙烯酰胺凝胶电泳 体积排阻色谱-高效液相色谱 鲎试剂法 酶联免疫吸附试验

生物特性参数 蛋白类型重组蛋白 种属来源Human 生物活性描述ELISA Data 1: Immobilized FITC-Labeled Human CD5 His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-CD5 Antibody hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. 应用CAR-T靶点蛋白

技术参数纯度> 95% as determined by SDS-PAGE and SEC-HPLC 内毒素水平Less than 1 EU per μg by the LAL method. 分子量The protein has a predicted MW of 39.63 kDa. Due to glycosylation the protein migrates to 50-60 kDa based on Bis-Tris PAGE result. 制剂形式Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. 活性ELISA Data 1: Immobilized FITC-Labeled Human CD5 His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-CD5 Antibody hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. 储存条件-25~-15℃保存，收到货之日起，有效期1年。复溶后， -85~-65℃保存，3个月有效期。避免反复冻融。 表达系统HEK293 标签C-His 外观Lyophilized Powder 复溶方法用蒸馏水溶解冻干的蛋白。如果规格为25μg或者100μg，建议复溶到0.5mg/mL的浓度。对于500μg或1mg的冻干粉，建议以1mg/mL的浓度复溶。复溶过程中请不要剧烈涡旋或者剧烈摇晃。将复溶后的蛋白溶液按照需求进行分装，储存于至-80℃，避免反复冻融。 表达序列Arg25-Asn371 蛋白登录号P06127 别名CD5 molecule;CD5;LEU1;T1;CD5 antigen