Recombinant Biotinylated Human IL-7 R alpha/CD127 Protein,His-Avi Tag

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HEK293表达系统抗体药物靶点研究白血病机制研究

Product no.96196ES Sizes3 options SourceYeasen · Official

Cat. No. 96196ES25

现货 $1,975.00

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96196ES25 25 μg $1,975.00 现货 Enquire

96196ES60 100 μg $7,885.00 现货 Enquire

96196ES76 500 μg $26,255.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No96196ES

Specification高活性、高纯度、低内毒素、高批间一致性

Origin翌圣生物科技(上海)股份有限公司

技术平台 HEK293表达系统

应用方法 抗体药物靶点研究作为IL-7R alpha/CD127靶点蛋白用于抗体药物筛选与开发 白血病机制研究研究IL-7/IL-7R信号通路对白血病细胞存活、周期和生长的促进作用

适用物种种属人

检测方法 SDS-PAGE SEC-HPLC Bis-Tris PAGE SPR LAL

生物特性参数 蛋白类型Recombinant Biotinylated Human IL-7 R alpha/CD127 Protein His-Avi Tag 种属来源Human 生物活性描述SPR Data: Biotinylated Human IL-7R alpha His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IL-7 No Tag with affinity constant of 7.37 nM as determined in SPR assay. IL-7/IL-7R activates STAT5 PI3K/Akt/mTOR and MEK/Erk pathways promoting leukemia cell viability cell cycle progression and growth. 应用抗体药物靶点研究；白血病机制研究

样本类型 重组蛋白

技术参数纯度>95% as determined by SDS-PAGE and SEC-HPLC 内毒素水平Less than 1 EU per μg by the LAL method 分子量Predicted MW of 28.16 kDa; due to glycosylation migrates to 50-65 kDa by Bis-Tris PAGE 制剂形式Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. 活性Binds Human IL-7 with affinity constant of 7.37 nM in SPR assay 储存条件-25~-15℃保存，收到货之日起有效期1年；复溶后-85~-65℃保存，3个月有效期；避免反复冻融。 复溶方法用蒸馏水溶解冻干的蛋白。25μg或100μg规格建议复溶到0.5mg/mL；500μg或1mg规格建议以1mg/mL复溶。复溶过程中避免剧烈涡旋或摇晃，分装后-80℃保存，避免反复冻融。外观Lyophilized Powder 表达系统HEK293 标签C-His-Avi AccessionP16871-1 表达序列Glu21-Asp239 产品别称IL-7R alpha;IL7Ra;CD127;CDW127;IL7Rα