Recombinant Biotinylated Human IL-18RAP/IL-18 R beta Protein,His-Avi Tag

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HEK293细胞表达系统抗体药物靶点蛋白免疫检测

Product no.96194ES Sizes3 options SourceYeasen · Official

Cat. No. 96194ES25

现货 $1,975.00

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96194ES25 25 μg $1,975.00 现货 Enquire

96194ES60 100 μg $7,885.00 现货 Enquire

96194ES76 500 μg $26,255.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No96194ES

Specification高活性、高纯度、低内毒素、高批间一致性

Origin翌圣生物科技(上海)股份有限公司

技术平台 HEK293细胞表达系统

应用方法 抗体药物靶点蛋白作为IL-18RAP靶点蛋白用于抗IL-18RAP抗体的筛选、活性评价及药物研发 免疫检测作为抗原或标准品用于ELISA等免疫检测

适用物种种属人

检测方法 ELISA SDS-PAGE SEC-HPLC LAL法 Bis-Tris PAGE

生物特性参数 蛋白类型重组蛋白（IL-18RAP/IL-18 R beta） 种属来源人（Human） 生物活性描述Immobilized Biotinylated Human IL-18 RAP His Avi Tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-IL-18RAP Antibody hFc Tag with the EC50 of 6.4ng/ml determined by ELISA. 应用抗体药物靶点蛋白，可用于ELISA等免疫检测

样本类型 重组蛋白

技术参数 内毒素水平Less than 1 EU per μg by the LAL method. 纯度> 95% as determined by SDS-PAGE and SEC-HPLC 分子量The protein has a predicted MW of 41.35 kDa. Due to glycosylation the protein migrates to 60-70 kDa based on Bis-Tris PAGE result. 制剂形式Supplied as 0.22 μm filtered solution in PBS (pH 7.4). pH7.4 表达系统HEK293 标签C-His-Avi 生物素化Biotinylated 表达序列Phe20-Arg356 Accession号O95256-1 外观Liquid 活性ELISA测定Anti-IL-18RAP Antibody hFc Tag的EC50为6.4 ng/ml 储存条件-85~-65℃保存，收到货之日起，有效期1年。建议分装蛋白为一次实验使用量，分装后，-80℃冻存。避免反复冻融。