Recombinant Biotinylated Human VEGF165 Protein,His-Avi Tag

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HEK293表达系统抗体药物研发

Product no.93713ES Sizes3 options SourceYeasen · Official

Cat. No. 93713ES60

现货 ¥6,885.00

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93713ES60 100 μg ¥6,885.00 现货 Enquire

93713ES76 500 μg ¥24,115.00 现货 Enquire

93713ES25 25 μg ¥2,285.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No93713ES

Specification高纯度、高活性、低内毒素、高批间一致性

Origin翌圣生物科技(上海)股份有限公司

技术平台 HEK293表达系统

应用方法 抗体药物研发靶点蛋白

适用物种种属人

检测方法 SDS-PAGE LAL法 ELISA

生物特性参数 蛋白类型重组生物素化蛋白 种属来源人 生物活性描述Immobilized Anti-VEGF165 Antibody at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Human VEGF165 His Tag with the EC50 of 13.7 ng/ml determined by ELISA. 应用抗体药物靶点研究

技术参数纯度> 95% as determined by SDS-PAGE. 内毒素水平< 1.0 EU per μg by the LAL method. 分子量Predicted MW 22.2 kDa. Due to glycosylation migrates to 28-35 kDa under reduced (R) condition 45-60 kDa under non-reducing (N) condition by SDS-PAGE. 制剂形式Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. 活性EC50 = 13.7 ng/ml determined by ELISA. 储存条件-25 ~ -15℃保存，收到货之后有效期1年。复溶后，无菌条件下，-85 ~ -65℃保存，3个月有效期。 表达系统HEK293 标签C-His-Avi 表达序列Ala27-Arg191 复溶方法开盖之前，先离心。使用蒸馏水复溶冻干的蛋白质，复溶浓度超过100 μg/mL。建议首次使用时将蛋白分装为每次实验用量，避免反复冻融。 外观Lyophilized Powder Accession号P15692-4