Recombinant Human PVRIG (mFc Tag)

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HEK293表达系统免疫检查点研究抗体筛选配体结合研究

Product no.93570ES Sizes3 options SourceYeasen · Official

Cat. No. 93570ES60

现货 ¥4,655.00

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93570ES60 100 μg ¥4,655.00 现货 Enquire

93570ES76 500 μg ¥14,685.00 现货 Enquire

93570ES80 1 mg ¥31,135.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No93570ES

Specification高纯度、高活性、低内毒素、高批间一致性

Origin翌圣生物科技(上海)股份有限公司

技术平台 HEK293表达系统

应用方法 免疫检查点研究研究PVRIG在免疫调节中的作用 抗体筛选用于筛选抗PVRIG抗体及其结合活性评价 配体结合研究研究PVRIG与Nectin-2/CD112的相互作用

适用物种种属人

检测方法 酶联免疫吸附试验 表面等离子共振 聚丙烯酰胺凝胶电泳 高效液相色谱

生物特性参数 蛋白类型重组蛋白 种属来源人 生物活性描述1. ELISA: Immobilized Human PVRIG at 1μg/mL (100μL/well) on the plate. Dose response curve for Anti-PVRIG Antibody hFc Tag with the EC50 of 8.0 ng/mL. 2. ELISA: Immobilized Human PVRIG mFc Tag at 1μg/mL (100μL/well) on the plate. Dose response curve for Human Nectin-2 hFc Tag with the EC50 of 16.9 ng/mL. 3. SPR: Human PVRIG mFc Tag captured on CM5 Chip via Anti-Mouse Antibody can bind Human Nectin-2 His Tag with an affinity constant of 0.349 μM as determined in SPR assay. 应用免疫检查点研究，抗体筛选，配体结合研究

技术参数纯度> 95% as determined by SDS-PAGE and HPLC 内毒素水平< 1.0 EU per μg by the LAL method 分子量Approximately 39.6 kDa; due to glycosylation migrates to 50-65 kDa based on Tris-Bis PAGE result 制剂形式Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization 储存条件-25 ~ -15℃保存，收到货之后有效期1年；复溶后，无菌条件下，-85 ~ -65℃保存，3个月有效期 表达系统HEK293 标签C-mFc 表达序列Thr41-Asp171 外观Lyophilized Powder 复溶方法开盖之前，先离心。使用蒸馏水复溶冻干的蛋白质，复溶浓度超过100 μg/mL。建议首次使用时将蛋白分装为每次实验用量，避免反复冻融。 登录号Q6DKI7-1 产品别称C7orf15 CD112R MGC104322 MGC138297 MGC2463 PVRIG ALS2CR18 ALS2CR9 LPD PREL-2 PREL2 RalGDS AF-6 RMO1