Hieff UNICON® qPCR SYBR Green Master Mix ( 抗体法，High Rox)

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实时荧光定量PCR染料法荧光定量PCR

Product no.11200ES Sizes4 options Documents1 files SourceYeasen · Official

Cat. No. 11200ES03

现货 ¥336.00

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Cat.No Spec Price Status

11200ES03 1 mL ¥336.00 现货 Enquire

11200ES08 5× 1 mL ¥588.00促~~¥1,466.00~~ 现货 Enquire

11200ES50 50× 1 mL ¥11,086.00 现货 Enquire

11200ES60 100× 1 mL ¥10,095.00促~~¥17,596.00~~ 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No11200ES

Specification特异性好

Origin翌圣生物科技(上海)股份有限公司

技术平台 实时荧光定量PCR

应用方法 染料法荧光定量PCR利用SYBR Green I染料检测PCR产物积累进行定量

检测方法 SYBR Green I染料法

生物特性参数 试剂类型qPCR预混液 应用染料法荧光定量PCR

技术参数 产品形式预混液 热启动抗体法热启动 ROXHigh Rox 扩增长度80-300bp 适配型号High Rox机型 缓冲液组分含Hieff Taq DNA聚合酶、UNICON Taq抗体、SYBR Green I、dNTPs、Mg2+、High Rox 储存条件-20℃避光储存，有效期18个月，避免反复冻融 运输条件冰袋运输

实验类型 qPCR实时荧光定量PCR扩增与核酸定量分析

FAQ

Q：最近做相对定量实验，Ct 值一直在 30 以后，是否可以考虑用这一款产品?

A：可以，这款是高灵敏度的，针对低丰度样本开发优化的。

Documents & Citations

实时荧光定量PCR实验技术手册(反转定量) Open document

Published citations

[1] Yan P, Li Z, Xiong J, et al. LARP7 ameliorates cellular senescence and aging by allosterically enhancing SIRT1 deacetylase activity. Cell Rep. 2021;37(8):110038. doi:10.1016/j.celrep.2021.110038(IF:9.423)

[2] Huang C, Zhang N, Xiong H, et al. Multi-Omics Analysis for Transcriptional Regulation of Immune-Related Targets Using Epigenetic Data: A New Research Direction. Front Immunol. 2022;12:741634. Published 2022 Jan 3. doi:10.3389/fimmu.2021.741634(IF:7.561)

[3] Ni Z, Huang C, Zhao H, et al. PLXNC1: A Novel Potential Immune-Related Target for Stomach Adenocarcinoma. Front Cell Dev Biol. 2021;9:662707. Published 2021 Jul 2. doi:10.3389/fcell.2021.662707(IF:6.684)

[4] Huang C, Huang R, Chen H, et al. Chromatin Accessibility Regulates Gene Expression and Correlates With Tumor-Infiltrating Immune Cells in Gastric Adenocarcinoma. Front Oncol. 2021;10:609940. Published 2021 Jan 5. doi:10.3389/fonc.2020.609940(IF:6.244)

[5] Wang Y, Chen Z, Zhang X, et al. Single-Base Resolution Mapping Reveals Distinct 5-Formylcytidine in Saccharomyces cerevisiae mRNAs. ACS Chem Biol. 2022;17(1):77-84. doi:10.1021/acschembio.1c00633(IF:5.100)

[6] Jian W, Deng XC, Munankarmy A, et al. KIF23 promotes triple negative breast cancer through activating epithelial-mesenchymal transition. Gland Surg. 2021;10(6):1941-1950. doi:10.21037/gs-21-19(IF:2.953)

[7] Lv J, Liu W, Shi G, et al. Human cardiac extracellular matrix-chitosan-gelatin composite scaffold and its endothelialization. Exp Ther Med. 2020;19(2):1225-1234. doi:10.3892/etm.2019.8349(IF:1.448)

[8] Cai Y, Zheng Q, Yao DJ. Phosphatidylinositol-3,4,5-trisphosphate dependent Rac exchange factor 1 is a diagnostic and prognostic biomarker for hepatocellular carcinoma. World J Clin Cases. 2020;8(17):3774-3785. doi:10.12998/wjcc.v8.i17.3774(IF:1.013)