Recombinant Biotinylated Human Latent TGF beta 1/TGFB1 Protein,His-Avi Tag

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HEK293表达系统抗体药物靶点研究

Product no.93532ES Sizes3 options SourceYeasen · Official

Cat. No. 93532ES60

现货 ¥8,235.00

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93532ES60 100 μg ¥8,235.00 现货 Enquire

93532ES76 500 μg ¥27,485.00 现货 Enquire

93532ES25 25 μg ¥2,265.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No93532ES

Specification高纯度、高活性、低内毒素、高批间一致性

Origin翌圣生物科技(上海)股份有限公司

技术平台 HEK293表达系统

应用方法 抗体药物靶点研究靶点蛋白用于抗体药物研发及筛选

适用物种种属人

检测方法 ELISA Tris-Bis PAGE HPLC LAL法

生物特性参数 蛋白类型Recombinant Biotinylated Human Latent TGF beta 1/TGFB1 Protein His-Avi Tag 种属来源Human 生物活性描述Immobilized Human ITGAV&ITGB8 His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Latent TGF beta 1 His Tag with EC50 of 0.39 μg/ml determined by ELISA.

技术参数纯度>95% as determined by Tris-Bis PAGE >93% as determined by HPLC 内毒素水平<1.0 EU per μg by the LAL method 分子量Predicted 44.2 kDa; due to glycosylation migrates to 40-50 (LAP)/13-15 (Mature TGF beta 1) kDa based on Tris-Bis PAGE 制剂形式Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. 活性EC50 of 0.39 μg/ml determined by ELISA (Immobilized Human ITGAV&ITGB8 His Tag at 5μg/ml) 储存条件-25 ~ -15℃保存，收到货后有效期1年；复溶后，无菌条件下，-85 ~ -65℃保存，3个月有效期。 表达系统HEK293 标签N-His-Avi AccessionP01137 表达序列Leu30-Ser390(C33S) 复溶方法开盖之前，先离心。使用蒸馏水复溶冻干的蛋白质，复溶浓度超过100 μg/mL。建议首次使用时将蛋白分装为每次实验用量，避免反复冻融。 外观Lyophilized Powder