Recombinant Biotinylated Human Fc gamma RIIB/CD32b Protein,His-Avi Tag

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HEK293细胞表达系统抗体药物靶点研究

Product no.93460ES Sizes3 options SourceYeasen · Official

Cat. No. 93460ES60

现货 ¥6,885.00

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93460ES60 100 μg ¥6,885.00 现货 Enquire

93460ES76 500 μg ¥24,115.00 现货 Enquire

93460ES25 25 μg ¥2,285.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No93460ES

Specification高纯度、高活性、低内毒素、高批间一致性

Origin翌圣生物科技(上海)股份有限公司

技术平台 HEK293细胞表达系统

应用方法 抗体药物靶点研究作为靶点蛋白用于抗体药物筛选与开发

适用物种种属人

检测方法 SDS-PAGE HPLC SPR LAL

生物特性参数 蛋白类型重组蛋白（Recombinant Biotinylated Human Fc gamma RIIB/CD32b His-Avi Tag） 种属来源人 生物活性描述通过SPR测定，与Rituximab结合的亲和常数分别为5.87 μM（Biotinylated Fc gamma RIIB捕获）和3.97 μM（Rituximab捕获）。 应用抗体药物靶点研究 Accession编号P31994-1 产品别称Fc gamma RII Fc gamma RIIB CD32 CD32b CDw32 FCG2 IGFR2 CD32A FCG2 FcGR FCGR2 FCGR21 FCGR2A

技术参数纯度> 95% as determined by SDS-PAGE and HPLC. 内毒素水平< 1.0 EU per μg by the LAL method. 分子量Predicted MW of 22.5 kDa; migrates to 32-37 kDa due to glycosylation (Tris-Bis PAGE). 制剂形式Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 活性SPR shows binding to Rituximab with affinity constants of 5.87 μM (Biotinylated Fc gamma RIIB captured) and 3.97 μM (Rituximab captured). 储存条件-25 ~ -15℃保存，收到货之后有效期1年。复溶后，无菌条件下，-85 ~ -65℃保存，3个月有效期。 表达系统HEK293 表达序列Ala46-Pro217 标签C-His-Avi 复溶方法开盖之前，先离心。使用蒸馏水复溶冻干的蛋白质，复溶浓度超过100 μg/mL。建议首次使用时将蛋白分装为每次实验用量，避免反复冻融。 外观Lyophilized Powder