Recombinant Human Fc gamma RIIIA/CD16a (F176) (His-Avi Tag)

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HEK293表达系统免疫学研究抗体药物研发

Product no.93445ES Sizes4 options SourceYeasen · Official

Cat. No. 93445ES60

现货 ¥3,385.00

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Cat.No Spec Price Status

93445ES60 100 μg ¥3,385.00 现货 Enquire

93445ES76 500 μg ¥11,305.00 现货 Enquire

93445ES80 1 mg ¥21,475.00 现货 Enquire

93445ES25 25 μg ¥1,185.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No93445ES

Specification高纯度、高活性、低内毒素、高批间一致性

Origin翌圣生物科技(上海)股份有限公司

技术平台 HEK293表达系统

应用方法 免疫学研究Fc受体功能与免疫应答机制研究 抗体药物研发ADCC效应功能评价

适用物种种属人

检测方法 SDS-PAGE HPLC ELISA SPR LAL Tris-Bis PAGE

生物特性参数 蛋白类型重组蛋白/靶点蛋白/Fc受体蛋白 种属来源Human 生物活性描述ELISA Data: Immobilized Human Fc gamma RIIIA (F176) His Tag at 2μg/ml (100μl/well) on the plate; dose response curve for Anti-Fc gamma RIIIA Antibody hFc Tag with EC50 of 9.5ng/ml. SPR DataHuman Fc gamma RIIIA (F176) His Tag captured on CM5 Chip via anti-His antibody can bind Rituximab hFc Tag with affinity constant of 0.92μM. 应用用于FcγRIIIa/CD16a相关的免疫应答、ADCC及抗体药物功能研究。

样本类型 重组蛋白

技术参数纯度>95% by SDS-PAGE and HPLC 内毒素水平<1.0 EU per μg by LAL method 分子量预测分子量24.7 kDa；因糖基化，Tris-Bis PAGE迁移至48-58 kDa 制剂形式Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 储存条件-25 ~ -15℃保存，收到货之后有效期1年。复溶后，无菌条件下，-85 ~ -65℃保存，3个月有效期。 表达系统HEK293 标签C-His-Avi 表达序列Gly17-Gln208(F176) AccessionP08637-1 外观Lyophilized Powder 复溶方法开盖之前，先离心。使用蒸馏水复溶冻干的蛋白质，复溶浓度超过100 μg/mL。建议首次使用时将蛋白分装为每次实验用量，避免反复冻融。