Recombinant Human SIRP alpha V2/CD172a Protein,His Tag

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Product no.93379ES Sizes3 options SourceYeasen · Official

Cat. No. 93379ES60

现货 $3,585.00

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93379ES60 100 μg $3,585.00 现货 Enquire

93379ES76 500 μg $12,355.00 现货 Enquire

93379ES25 25 μg $1,195.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No93379ES

Specification高纯度、高活性、低内毒素、高批间一致性

Origin翌圣生物科技(上海)股份有限公司

适用物种种属人

检测方法 SDS-PAGE 高效液相色谱 鲎试剂法 酶联免疫吸附测定 表面等离子体共振

生物特性参数 蛋白类型重组蛋白/靶点蛋白/抗体药物靶点蛋白 种属来源Human 生物活性描述【Blocking Data】Serial dilutions of Anti-SIRP alpha Antibody hFc Tag were added into Human SIRP alpha V2 His TagBiotinylated Human CD47 Protein His Tag binding reactioins.The half maximal inhibitiory concentration (IC50) is 54.14ng/ml. 【ELISA Data】Immobilized Human SIRP alpha V2 His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-SIRP alpha Antibody hFc Tag with the EC50 of 4.0ng/ml determined by ELISA.Immobilized Human SIRP alpha V2 His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human CD47 hFc Tag with the EC50 of 0.15μg/ml determined by ELISA. 【SPR Data】Human SIRP alpha V2 His Tag captured on Protein A Chip via Anti-His Antibody can bind Anti-SIRP alpha Ab. hFc Tag with an affinity constant of 9.11nM as determined in SPR assay.

样本类型 重组蛋白

技术参数纯度> 95% as determined by SDS-PAGE and HPLC. 内毒素水平< 1.0 EU per μg by the LAL method. 分子量The protein has a predicted MW of 38 kDa. Due to glycosylation the protein migrates to 55-65 kDa based on SDS-PAGE result. 制剂形式Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. 活性IC50=54.14ng/ml (Blocking) EC504.0ng/ml (ELISA) EC500.15μg/ml (ELISA) affinity9.11nM (SPR). 储存条件-25 ~ -15℃保存，收到货之后有效期2年。复溶后，无菌条件下，-85 ~ -65℃保存，3个月有效期。 表达序列Glu31-Arg369 表达系统HEK293 标签C-His 外观Lyophilized Powder 复溶方法开盖之前，先离心。使用蒸馏水复溶冻干的蛋白质，复溶浓度超过100 μg/mL。建议首次使用时将蛋白分装为每次实验用量，避免反复冻融。 AccessionAAH26692.1 产品别称CD172a MYD1 SHPS-1 SIRP alpha SIRPalpha2