Recombinant Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein, His Tag

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HEK293细胞免疫学研究抗体药物靶点

Product no.93348ES Sizes1 options SourceYeasen · Official

Cat. No. 93348ES25 现货 ¥1,455.00

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No93348ES

Specification高纯度、高活性、低内毒素、高批间一致性

Origin翌圣生物科技(上海)股份有限公司

技术平台 HEK293细胞

应用方法 免疫学研究研究CD3介导的T细胞活化机制 抗体药物靶点作为抗体药物靶点蛋白用于药物研发

适用物种种属人

检测方法 ELISA SPR SDS-PAGE HPLC

生物特性参数 蛋白类型重组蛋白/靶点蛋白/抗体药物靶点蛋白 种属来源人 生物活性描述与OKT3抗体结合，ELISA EC50=110.2 ng/ml；SPR亲和力=0.22 nM 应用T细胞活化研究、抗体药物开发 蛋白功能CD3是T细胞上的跨膜蛋白，与TCR形成复合物，参与MHC肽复合物诱导的T细胞活化

样本类型 重组蛋白

技术参数纯度> 95% as determined by SDS-PAGE and HPLC 内毒素水平< 1.0 EU per μg by the LAL method 分子量Predicted MW 17.9 kDa (CD3E) and 17.9 kDa (CD3G); due to glycosylation 25-40 kDa by Tris-Bis PAGE 制剂形式Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 活性ELISA: EC50=110.2 ng/ml; SPR: affinity constant=0.22 nM 储存条件-25 ~ -15℃保存，有效期1年；复溶后无菌条件下-85 ~ -65℃保存，3个月有效期 表达系统HEK293 标签C-His Accession号P07766(CD3E)&P09693(CD3G) 表达序列Asp23-Glu120(CD3E)&Gln23-Gln106(CD3G) 外观Lyophilized Powder 复溶方法使用蒸馏水复溶冻干的蛋白质，复溶浓度超过100 μg/mL；建议分装避免反复冻融 产品别称CD3 CD3G CD3g T3G CD3e CD3E CD3 epsilon&CD3 gamma CD3E&CD3G