Recombinant Cynomolgus B7-H3/CD276 Protein,His Tag

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HEK293表达系统CAR-T靶点研究

Product no.93219ES Sizes3 options SourceYeasen · Official

Cat. No. 93219ES60

现货 ¥3,585.00

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93219ES60 100 μg ¥3,585.00 现货 Enquire

93219ES76 500 μg ¥12,355.00 现货 Enquire

93219ES25 25 μg ¥1,195.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No93219ES

Specification高纯度、高活性、低内毒素、高批间一致性

Origin翌圣生物科技(上海)股份有限公司

技术平台 HEK293表达系统

应用方法 CAR-T靶点研究作为靶蛋白用于CAR-T细胞治疗研究

适用物种种属 食蟹猴

检测方法 ELISA法 SDS-PAGE电泳 高效液相色谱(HPLC) 鲎试剂法(LAL)

生物特性参数 蛋白类型重组蛋白 种属来源食蟹猴 生物活性描述ELISA Data: Immobilized Cynomolgus B7-H3 His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-B7-H3 Antibody hFc Tag with the EC50 of 11.1ng/ml determined by ELISA. 应用用于抗B7-H3抗体结合活性检测及CAR-T靶点研究 产品别称CD276 B7-H3 B7H3 UNQ309 AccessionXP_015308534.1

技术参数纯度>95% as determined by SDS-PAGE and HPLC. 内毒素水平<1.0 EU per μg by the LAL method. 分子量The protein has a predicted MW of 48.2 kDa. Due to glycosylation the protein migrates to 70-80 kDa based on SDS-PAGE result. 制剂形式Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. 活性ELISA Data: Immobilized Cynomolgus B7-H3 His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-B7-H3 Antibody hFc Tag with the EC50 of 11.1ng/ml determined by ELISA. 储存条件-25 ~ -15℃保存，收到货之后有效期2年。复溶后，无菌条件下，-85 ~ -65℃保存，3个月有效期。 表达序列Leu29-Glu465 标签C-His 表达系统HEK293 外观Lyophilized Powder 复溶方法开盖之前，先离心。使用蒸馏水复溶冻干的蛋白质，复溶浓度超过100 μg/mL。建议首次使用时将蛋白分装为每次实验用量，避免反复冻融。