Recombinant FITC-Labeled Human B7-H3/CD276 Protein,hFc Tag

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流式细胞术CAR-T靶点蛋白免疫调控研究

Product no.93202ES Sizes1 options SourceYeasen · Official

Cat. No. 93202ES60 现货 ¥8,935.00

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93202ES60 100 μg ¥8,935.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No93202ES

Specification高纯度、高活性、低内毒素、高批间一致性

Origin翌圣生物科技(上海)股份有限公司

技术平台 流式细胞术

应用方法 CAR-T靶点蛋白靶点结合活性检测 免疫调控研究研究B7-H3在T细胞活化及肿瘤免疫中的调控作用

适用物种种属人

检测方法 荧光检测

生物特性参数 蛋白类型重组蛋白 种属来源人 AccessionQ5ZPR3-2 产品别称B7H3 B7-H3 CD276 PSEC0249 UNQ309 PRO352 B7 homolog 3 CD276 生物活性描述B7-H3 is a member of the B7 family of cell surface ligands that regulate T cell activation and immune responses. B7-H3 is a negative regulator of T cell response and inhibits T cell proliferation. 应用2e5 of transient transfected anti- CD276 ScFv CAR-293 cells were stained with 0.1ug FITC-Labeled B7-H3/CD276 Fc Chimera Protein Human and unlable respectively (Fig. C and B) and non-transfected 293 cells were used as a control (Fig. A). FITC signal was used to evaluate the binding activity. 2e5 of transient transfected anti- CD276 ScFv CAR-293 cells were stained with isotype and Whitlow/218 Linker-Alexa Fluor® 488 (Fig. D and E). Alexa Fluor® 488 signal was used to evaluate the binding activity.

样本类型细胞

技术参数纯度> 95% as determined by SDS-PAGE. 内毒素水平< 1.0 EU per μg by the LAL method. 分子量60-70kDa 制剂形式Supplied as 0.22μm filtered solution in PBS (pH 7.4). 表达系统HEK293 标签C-hFc 表达序列Leu29-Pro245 with Human IgG1 Fc at the C-Terminus 外观Liquid 储存条件-85 ~ -65℃保存，收到货之后有效期1年。避免反复冻融，打开小瓶前先离心。建议等分分装为一次实验使用量，分装后，-80℃冻存。