Biotinylated Human Nectin-4 Protein IgV Domain, His-Avi Tag

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HEK293表达系统抗体药物研发ELISA检测

Product no.93134ES Sizes4 options SourceYeasen · Official

Cat. No. 93134ES60

现货 $6,885.00

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93134ES60 100 μg $6,885.00 现货 Enquire

93134ES76 500 μg $24,115.00 现货 Enquire

93134ES80 1 mg $43,865.00 现货 Enquire

93134ES25 25 μg $2,285.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No93134ES

Specification高纯度、高活性、低内毒素、高批间一致性

Origin翌圣生物科技(上海)股份有限公司

技术平台 HEK293表达系统

应用方法 抗体药物研发Nectin-4靶点蛋白 ELISA检测抗Nectin-4抗体结合活性检测

适用物种种属人

检测方法 SDS-PAGE HPLC ELISA LAL法 Tris-Bis PAGE

生物特性参数 蛋白类型重组蛋白/靶点蛋白 种属来源Human 生物活性描述Immobilized Biotinylated Human Nectin-4 IgV Domain His Tag at 0.5 μg/ml (100 μl/well) on streptavidin precoated plate (5 μg/ml); dose response curve for Anti-Nectin-4 Antibody hFc Tag with EC50 of 3.4 ng/ml determined by ELISA. 应用抗体药物靶点研究；ELISA免疫检测 别名EDSS1; LNIR; Nectin4; PRR4; NECTIN4; PVRL4 蛋白背景Nectin-4 is a 66 kDa type I transmembrane glycoprotein belonging to Nectin family of Ig superfamily proteins. Extracellular domain of human Nectin-4 shares 91% 92% 93% 91% and 90% amino acid sequence homology with mouse rat canine porcine and bovine Nectin-4. Upregulated in ductal breast or non-small cell lung carcinomas; involved in adherens junction formation and cell-cell interactions.

样本类型 重组蛋白

技术参数纯度> 95% as determined by SDS-PAGE and HPLC. 内毒素水平< 1.0 EU per μg by the LAL method. 分子量Predicted MW: 15.8 kDa; glycosylated protein migrates at 16-20 kDa by Tris-Bis PAGE. 制剂形式Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. 活性EC50 = 3.4 ng/ml (ELISA Anti-Nectin-4 Antibody hFc Tag) 储存条件-85 ~ -65°C保存，收到货后有效期1年；复溶后无菌条件下-85 ~ -65°C保存，有效期3个月。 复溶方法开盖前先离心；用蒸馏水复溶冻干蛋白，复溶浓度超过100 μg/mL；建议分装后避免反复冻融。 表达系统HEK293 标签C-His-Avi 表达序列Gly32-Leu146 AccessionQ96NY8-1 外观Lyophilized Powder