Recombinant Human B7-H3(4Ig)/B7-H3b (hFc Tag)

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HEK293表达系统CAR-T靶点蛋白

Product no.93111ES Sizes4 options SourceYeasen · Official

Cat. No. 93111ES25

现货 $1,195.00

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Cat.No Spec Price Status

93111ES25 25 μg $1,195.00 现货 Enquire

93111ES60 100 μg $3,585.00 现货 Enquire

93111ES76 500 μg $12,355.00 现货 Enquire

93111ES80 1 mg $24,155.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No93111ES

Specification高纯度、高活性、低内毒素、高批间一致性

Origin翌圣生物科技(上海)股份有限公司

技术平台 HEK293表达系统

应用方法 CAR-T靶点蛋白靶点研究

适用物种种属人

检测方法 ELISA SDS-PAGE HPLC LAL法 Tris-Bis PAGE

生物特性参数 蛋白类型重组蛋白 种属来源人 生物活性通过ELISA测定，固定Human B7-H3 (4Ig) hFc Tag于5μg/ml (100μl/Well)，生物素化Anti-B7-H3 Antibody hFc Tag的剂量反应曲线EC50为44.3ng/ml。 应用CAR-T靶点蛋白

样本类型 重组蛋白

技术参数 内毒素水平< 1.0 EU per μg by the LAL method. 分子量Approximately 73.4 kDa. Due to glycosylation the protein migrates to 80-110 kDa based on Tris-Bis PAGE result. 表达序列Gly27-Thr461 制剂形式Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally5% trehalose is added as protectant before lyophilization. 活性ELISA Data: Immobilized Human B7-H3 (4Ig) hFc Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-B7-H3 Antibody hFc Tag with the EC50 of 44.3ng/ml determined by ELISA. 产品别称B7-H3 CD276 PSEC0249 4Ig-B7-H3 UNQ309 PRO352 B7 homolog 3 CD276 复溶方法开盖之前，先离心。使用蒸馏水复溶冻干的蛋白质，复溶浓度超过100 μg/mL。建议首次使用时将蛋白分装为每次实验用量，避免反复冻融。 复溶浓度>100 μg/mL Accession号Q5ZPR3.1 纯度> 95% as determined by SDS-PAGE and HPLC. 表达系统HEK293 标签C-hFc 种属来源Human 外观Lyophilized Powder 储存条件-25 ~ -15℃保存，收到货之后有效期1年。复溶后，无菌条件下，-85 ~ -65℃保存，3个月有效期。 保护剂5% trehalose