HiActi® Recombinant Human TNF-alpha Protein,His tag 重组人肿瘤坏死因子-α

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大肠杆菌表达系统

Product no.90601ES Sizes4 options SourceYeasen · Official

Cat. No. 90601ES10

现货 ¥815.00

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90601ES10 10 μg ¥815.00 现货 Enquire

90601ES60 100 μg ¥2,985.00 现货 Enquire

90601ES76 500 μg ¥9,985.00 现货 Enquire

90601ES50 50 μg ¥1,985.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No90601ES

Specification高纯度、高活性、低内毒素、高批间一致性

Origin翌圣生物科技(上海)股份有限公司

技术平台 大肠杆菌表达系统

适用物种种属人

检测方法 SDS-PAGE 细胞毒性实验

生物特性参数 蛋白类型TNF-alpha 种属来源人 生物活性描述Measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D ED50 < 0.12 ng/ml.

技术参数纯度> 95% as determined by SDS-PAGE. 内毒素水平< 0.01 EU per μg by the LAL method. 分子量Approximately 18.5 kDa. 制剂形式20mM Tris 150mM NaCl 2mM TCEP pH8.5 储存条件存储温度：-25 ~ -15℃保存，收到货之后有效期1年。复溶后，无菌条件下，-85 ~ -65℃保存，3个月有效期。 表达系统E.coli 标签N-His 外观Lyophilized Powder 表达序列Val77-Leu233 复溶方法开盖之前，先离心，以将内容物带到底部。使用PBS复溶，重新配制至0.1-1.0 mg/mL的浓度，可根据后续实验更进一步稀释；长期储存，建议在稀释缓冲液中加入载体蛋白(0.1% BSA); 分装为一次实验用量，-80℃冻存，避免反复冻融。 Accession号P01375

实验类型 SDS-PAGE纯度检测 细胞毒性实验生物活性检测

Documents & Citations

Published citations

[1]. Shenghong W, Peilan G, Wenqiang M, et al. Liquid-liquid phase separation of ATXN2L enhances mRNA translation in hepatocellular carcinoma. Cell Reports,2025. https://doi.org/10.1016/j.celrep. (IF=9.9)