HiActi® Recombinant Mouse IFN-alpha 1a ,His Tag 重组小鼠干扰素IFN-α1a

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免疫学研究

Product no.91232ES Sizes4 options SourceYeasen · Official

Cat. No. 91232ES08

现货 $885.00

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Cat.No Spec Price Status

91232ES08 5 μg $885.00 现货 Enquire

91232ES20 20 μg $2,215.00 现货 Enquire

91232ES60 100 μg $6,885.00 现货 Enquire

91232ES76 500 μg $19,885.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No91232ES

Specification高活性、高纯度、低内毒素、高批间一致性

Origin翌圣生物科技(上海)股份有限公司

应用方法 免疫学研究研究干扰素介导的免疫信号转导

适用物种种属小鼠

检测方法 SDS-PAGE LAL法 细胞保护实验

生物特性参数 蛋白类型重组小鼠干扰素IFN-alpha 1a 种属来源小鼠 生物活性描述保护表达EMC病毒的L929细胞，ED50 <10 pg/mL；比活性>4 x 10^7 IU/mg。 产品别称interferon alpha 1a Ifa Ifa8 IFNA 登录号P01572 表达序列CDLPQTHNLRNKRALTLLVQMRRLSPLSCLKDRKDFGFPQEKVDAQQIKKAQAIPVLSELTQQILNIFTSKDSSAAWNTTLLDSFCNDLHQQLNDLQGCLMQQVGVQEFPLTQEDALLAVRKYFHRITVYLREKKHSPCAWEVVRAEVWRALSSSANVLGRLREEK with polyhistidine tag at the N-terminus.

技术参数纯度>98% as determined by SDS-PAGE. 内毒素水平<0.1 EU per 1 μg of the protein by the LAL method. 分子量Calculated MW: 19.93 kDa; migrates as 17-25 kDa under reducing condition. 制剂形式Lyophilized from a 0.2 µm filtered solution containing 1X PBS pH 7.4. 活性ED50 <10 pg/mL; specific activity >4 x 10^7 IU/mg. 储存条件-25~-15℃保存，有效期1年；复溶后2~8℃保存7天；复溶后-85~-65℃保存3个月。建议首次使用时等分，避免重复冻融循环。 表达系统E.coli 标签N-His 外观Lyophilized Powder 复溶方法打开前3000 rpm离心5分钟；用无菌水重悬至≥100 μg/mL，静置至少20min；可进一步稀释分装，需添加载体蛋白（0.1%BSA、10%FBS或5%HSA），无血清可用5%海藻糖。