HiActi® Recombinant Biotinylated Human IL-6 Protein,His-Avi Tag

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HEK293细胞表达系统AviTag生物素标记系统急性期反应炎症造血骨代谢癌症进展

Product no.90329ES Sizes3 options Documents1 files SourceYeasen · Official

Cat. No. 90329ES25

现货 ¥1,975.00

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90329ES25 25 μg ¥1,975.00 现货 Enquire

90329ES60 100 μg ¥7,885.00 现货 Enquire

90329ES76 500 μg ¥26,255.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No90329ES

Specification高活性、高纯度、低内毒素、高批间一致性

Origin翌圣生物科技(上海)股份有限公司

技术平台 HEK293细胞表达系统 AviTag生物素标记系统

应用方法 急性期反应研究IL-6在急性期反应中的作用 炎症研究IL-6在炎症反应中的作用 造血研究IL-6在造血过程中的作用 骨代谢研究IL-6在骨代谢中的作用 癌症进展研究IL-6在癌症进展中的作用

适用物种种属人

检测方法 SDS-PAGE SEC-HPLC Bis-Tris PAGE ELISA LAL法

生物特性参数 蛋白类型重组蛋白/细胞因子 种属来源人 生物活性描述ELISA Data 1: Immobilized Human IL-6 R alpha hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-6 His-Avi Tag with the EC50 of 0.18μg/ml determined by ELISA. 应用急性期反应、炎症、造血、骨代谢、癌症进展研究

技术参数纯度> 95% as determined by SDS-PAGE and SEC-HPLC 内毒素水平Less than 1 EU per μg by the LAL method. 分子量Predicted MW of 23.72 kDa; due to glycosylation migrates to 25-30 kDa based on Bis-Tris PAGE result. 制剂形式Lyophilized from 0.22 μm filtered solution in PBS 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. 储存条件-25~-15℃保存，有效期1年；复溶后-85~-65℃保存，3个月；避免反复冻融。 表达系统HEK293 标签N-His-Avi 标记Biotinylated 表达序列Val30-Met212 AccessionP05231 复溶方法用蒸馏水溶解冻干的蛋白。如果规格为25μg或者100μg，建议复溶到0.5mg/mL的浓度。对于500μg或1mg的冻干粉，建议以1mg/mL的浓度复溶。复溶过程中请不要剧烈涡旋或者剧烈摇晃。将复溶后的蛋白溶液按照需求进行分装，储存于至-80℃，避免反复冻融。外观Lyophilized Powder 活性ELISA EC50 of 0.18 μg/ml determined by ELISA