HiActi® Recombinant Mouse IL-28B Protein ,His Tag 重组小鼠白介素-28B

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大肠杆菌表达系统抗病毒研究

Product no.90294ES Sizes4 options SourceYeasen · Official

Cat. No. 90294ES08

现货 ¥885.00

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90294ES08 5 μg ¥885.00 现货 Enquire

90294ES20 20 μg ¥2,215.00 现货 Enquire

90294ES60 100 μg ¥6,885.00 现货 Enquire

90294ES76 500 μg ¥19,885.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No90294ES

Specification高活性、高纯度、低内毒素、高批间一致性

Origin翌圣生物科技(上海)股份有限公司

技术平台 大肠杆菌表达系统

应用方法 抗病毒研究IL-28B参与抗病毒免疫防御，可用于抗病毒机制研究

适用物种种属小鼠

检测方法 SDS-PAGE LAL法 细胞病变抑制实验

生物特性参数 蛋白类型重组蛋白/细胞因子 种属来源小鼠 生物活性描述Measure by its ability to protect HepG2 cells infected with encephalomyocarditis (EMC) virus. The ED₅₀ for this effect is <5 ng/mL. 应用抗病毒免疫防御研究

样本类型 重组蛋白

技术参数纯度>98% as determined by SDS-PAGE. 内毒素水平<0.1 EU per 1 μg of the protein by the LAL method. 分子量The protein has a calculated MW of 25.57 kDa. The protein migrates as 21 kDa under reducing condition. 制剂形式The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS pH 8.0. 活性Measure by its ability to protect HepG2 cells infected with encephalomyocarditis (EMC) virus. The ED₅₀ for this effect is <5 ng/mL. 储存条件-25 ~ -15℃保存，收到货之后有效期1年。复溶后，2~8°C保存，7天有效期。复溶后，-85~-65℃保存，3个月有效期。建议首次使用时将蛋白等分，避免重复冻融循环。规格高活性、高纯度、低内毒素、高批间一致性 分类细胞因子/白介素/IL-28 表达序列MVPVARLRGALPDARGCHIAQFKSLSPQELQAFKRAKDALEESLLLKDCKCRSRLFPRTWDLRQLQVRERPVALEAELALTLKVLEATADTDPALGDVLDQPLHTLHHILSQLRACIQPQPTAGPRTRGRLHHWLHRLQEAPKKESPGCLEASVTFNLFRLLTRDLNCVASGDLCV with polyhistidine tag at the C-terminus. 表达系统E.coli 标签C-His 外观Lyophilized Powder AccessionQ8IZI9 产品别称interleukin 28B interferon lambda 3 IFNL3 IFN-lambda-3 IFN-lambda-4 IL28B 复溶方法1.打开前，以3000 rpm离心5分钟。2.推荐使用无菌水重悬冻干粉，溶液浓度不低于100 μg/mL，并静置至少20min以充分溶解。3.重悬后的溶液可进一步稀释分装。进一步稀释分装时，须添加一定量的载体蛋白（0.1%BSA、10%FBS 或 5%HSA），无血清实验需求时，可更换为 5%海藻糖溶液作为载体。