HiActi® Recombinant Mouse IL-27 EBI3 Protein ,His Tag 重组小鼠白介素-27 EBI3

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大肠杆菌表达系统

Product no.90292ES Sizes4 options SourceYeasen · Official

Cat. No. 90292ES08

现货 ¥885.00

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Cat.No Spec Price Status

90292ES08 5 μg ¥885.00 现货 Enquire

90292ES20 20 μg ¥2,215.00 现货 Enquire

90292ES60 100 μg ¥6,885.00 现货 Enquire

90292ES76 500 μg ¥19,885.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No90292ES

Specification高活性、高纯度、低内毒素、高批间一致性

Origin翌圣生物科技(上海)股份有限公司

技术平台 大肠杆菌表达系统

适用物种种属小鼠

检测方法 SDS-PAGE LAL法

生物特性参数 蛋白类型重组蛋白 种属来源小鼠 生物活性描述能够保护感染脑心肌炎病毒(EMC)的HepG2细胞，ED50<5 ng/mL。

技术参数 内毒素水平<0.1 EU per 1 μg of the protein by the LAL method. 分子量The protein has a calculated MW of 23.84 kDa. The protein migrates about 25 kDa under reducing condition 纯度>98% as determined by SDS-PAGE. 表达序列MALVALSQPRVQCHASRYPVAVDCSWTPLQAPNSTRSTSFIATYRLGVATQQQSQPCLQRSPQASRCTIPDVHLFSTVPYMLNVTAVHPGGASSSLLAFVAERIIKPDPPEGVRLRTAGQRLQVLWHPPASWPFPDIFSLKYRLRYRRRGASHFRQVGPIEATTFTLRNSKPHAKYCIQVSAQDLTDYGKPSDWSLPGQVESAPHKP with polyhistidine tag at the C-terminus. 制剂形式The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS pH 7.4. 活性Measure by its ability to protect HepG2 cells infected with encephalomyocarditis (EMC) virus. The ED₅₀ for this effect is <5 ng/mL. 复溶方法1.打开前，以3000 rpm离心5分钟。 2.推荐使用无菌水重悬冻干粉，溶液浓度不低于100 μg/mL，并静置至少20min以充分溶解。 3.重悬后的溶液可进一步稀释分装。进一步稀释分装时，须添加一定量的载体蛋白（0.1%BSA、10%FBS 或 5%HSA），无血清实验需求时，可更换为 5%海藻糖溶液作为载体。 Accession号O35228 表达系统E.coli 标签C-His 外观Lyophilized Powder 储存条件-25 ~ -15℃保存，收到货之后有效期1年。复溶后，2 ~ 8 °C保存，7天有效期。复溶后，-85 ~ -65℃保存，3个月有效期。建议首次使用时将蛋白等分，避免重复冻融循环。 pH7.4 批间一致性高