HiActi® Recombinant Mouse IL-15 Protein,His Tag 重组小鼠白介素-15

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免疫调节

Product no.90218ES Sizes3 options SourceYeasen · Official

Cat. No. 90218ES20

现货 $1,935.00

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90218ES20 20 μg $1,935.00 现货 Enquire

90218ES50 50 μg $3,485.00 现货 Enquire

90218ES60 100 μg $5,535.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No90218ES

Specification高纯度、高活性、低内毒素、高批间一致性

Origin翌圣生物科技(上海)股份有限公司

应用方法 免疫调节T淋巴细胞增殖和NK细胞活化研究

适用物种种属小鼠

检测方法 SDS-PAGE LAL法

生物特性参数 蛋白类型重组蛋白 种属来源小鼠 生物活性描述Measure by its ability to induce CTLL-2 cells proliferation. The ED50 for this effect is <10 ng/mL. The specific activity of recombinant mouse IL-15 is approximately >1x 10⁵ IU/mg. 应用免疫调节/细胞因子研究

技术参数纯度> 95% as determined by SDS-PAGE. 内毒素水平< 1.0 EU per μg by the LAL method. 分子量The protein has a calculated MW of 14.2 kDa. The protein migrates as 17 kDa under reducing condition (SDS-PAGE analysis). 制剂形式Lyophilized from a 0.22 μm filtered solution of 1×PBS pH 7.4. 活性ED50 <10 ng/mL; specific activity >1x10⁵ IU/mg 储存条件-25 ~ -15℃保存，收到货之后有效期1年。复溶后，2 ~ 8°C保存，7天有效期。复溶后，-85 ~ -65℃保存，3个月有效期。建议首次使用时将蛋白等分，避免重复冻融循环。 表达系统E.coli 标签N-His 表达序列Asn49-Ser162 外观Lyophilized Powder 复溶方法开盖之前，以3000 rpm 离心 5 分钟。建议将冻干蛋白质在无菌 H2O 中重新配制至浓度不低于 100μg/mL，并在室温下孵育储备溶液至少 20 分钟，以确保充分再溶解。不要漩涡！剧烈摇晃可能会损害蛋白质的生物活性。如需长期储存，建议添加载体蛋白或稳定剂（例如 0.1%BSA、5%HSA、10%FBS 或 5%海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，避免反复冻融。 AccessionP48346

实验类型 CTLL-2增殖实验生物活性检测

Documents & Citations

Published citations

[1] Songbo Zhao,Rongkun Li ,Yuan Xia, et al.Targeting ECM-producing cells with CAR-T therapy alleviates fibrosis in chronic kidney disease.Cell Stem Cell. 2025 Aug 18:S1934-5909(25)00271-1. doi: 10.1016/j.stem.2025.07.014. ( IF:22)