Tick the specs you need to submit them together; or enquire one by one. Cat. numbers are carried into the RFQ.
Q:该产品可主要用于哪些样品?
A:本品适用的范围广泛,可应用于细胞裂解液、细胞分泌液上清、血清、动物腹水以及其它的免疫抗原等样本。
Q:工作液应配置多少浓度?
A:工作液浓度应根据具体实验确定,建议进行预实验摸索最佳实验浓度。
Q:血清样品可以稀释吗?
A:可以。若目标蛋白丰度较高,建议用结合缓冲液稀释血清样品至目标蛋白终浓度为10-100µg/mL,置于冰上备用(或置于-20℃长期保存)。
Q:抗原变性洗脱的样品可以用于 SDS-PAGE 检测吗?
A:可以。
Q:说明书中COIP实验操作中取适量磁珠是要加多少量?
A:一般磁珠的量是根据自己要做的样品的量决定。最小的常用COIP体系为:20ul磁珠混悬液+5ug抗体+总体积500ul 总蛋白浓度1mg/ml的裂解液混合孵育
Q:磁珠可以结合哪些抗体,结合力怎么样?
A:参照下表
蛋白A,G对不同物种Ig的结合能力总表
| 免疫球蛋白亚型 | Protein A | Protein G | 免疫球蛋白亚型 | Protein A | Protein G |
| Human IgG | ++++ | ++++ | Mouse IgG | ++++ | ++++ |
| Human IgG1 | ++++ | ++++ | Mouse IgG1 | + | ++++ |
| Human IgG2 | ++++ | ++++ | Mouse IgG2a | ++++ | ++++ |
| Human IgG3 | + | ++++ | Mouse IgG2b | +++ | +++ |
| Human IgG4 | ++++ | ++++ | Mouse IgG3 | ++ | +++ |
| Human IgM | Use anti-Human IgM | Mouse IgM | Use anti-Mouse IgM | ||
| Human IgE | NR | NR | Chicken IgG (IgY) | NR | NR |
| Human IgA | + | NR | Cow IgG | ++ | ++++ |
| Human IgA1 | + | NR | Goat IgG | + | ++++ |
| Human IgA2 | + | NR | Goat IgG1 | + | ++++ |
| Human IgD | Use anti-Human IgD | Goat IgG2 | ++++ | ++++ | |
| Rat IgG | + | ++ | Goat IgM | NR | NR |
| Rat IgG1 | NR | + | Guinea Pig IgG | ++++ | ++ |
| Rat IgG2a | NR | NR | Guinea Pig IgG1 | ++++ | ++ |
| Rat IgG2b | NR | + | Guinea Pig IgG2 | ++++ | ++ |
| Rat IgG3 | + | ++ | Hamster IgG | + | ++ |
| Sheep IgG | + | ++ | Horse IgG | + | ++++ |
| Sheep IgG1 | + | ++ | Rabbit IgG | ++++ | +++ |
| Sheep IgG2 | + | ++ | Rabbit IgM | NR | NR |
| Sheep IgM | NR | NR | Rabbit All isotypes | +++ | ++ |
| Pig IgG | +++ | +++ | Monkey IgG | ++++ | ++++ |
| Cat IgG | ++++ | + | Donkey IgG | ++ | ++++ |
| Dog IgG | ++++ | + | |||
| (+)= weak binding;(++)= moderate binding;(++++)= strong binding;NR= not recommended; (-)= not tested; | |||||
Q:磁珠可以重复利用吗?
A:不建议重复使用。
Q:变性洗脱得到的是抗体-蛋白的复合物吗?磁珠上的蛋白A、G会不会洗下来?
A:变性洗脱得到的是抗体-抗体复合物;蛋白是直接偶联到磁珠上,变性洗脱条件不会把A/G洗下来。
Published citations
[1] Jiang Y, Guo H, Tong T, et al. lncRNA lnc-POP1-1 upregulated by VN1R5 promotes cisplatin resistance in head and neck squamous cell carcinoma through interaction with MCM5. Mol Ther. 2022;30(1):448-467. doi:10.1016/j.ymthe.2021.06.006(IF:11.454)
[2] Cen Y, Zou X, Zhong Q, et al. The TIAR-mediated Nrf2 response to oxidative stress is mediated through the Nrf2 noncoding 3'untranslated region in Spodoptera litura. Free Radic Biol Med. 2022;184:17-29. doi:10.1016/j.freeradbiomed.2022.03.016(IF:7.376)
[3] Zhenzhen L, Wenting L, Jianmin Z, et al. miR-146a-5p/TXNIP axis attenuates intestinal ischemia-reperfusion injury by inhibiting autophagy via the PRKAA/mTOR signaling pathway. Biochem Pharmacol. 2022;197:114839. doi:10.1016/j.bcp.2021.114839(IF:5.858)
[4] Meng J, Zhang C, Wang D, Zhu L, Wang L. Mitochondrial GCN5L1 regulates cytosolic redox state and hepatic gluconeogenesis via glycerol phosphate shuttle GPD2 [published online ahead of print, 2022 Jun 28]. Biochem Biophys Res Commun. 2022;621:1-7. doi:10.1016/j.bbrc.2022.06.092(IF:3.575)
[5] Chen P, Wang L, Long YB, et al. E2F4 regulates the cell cycle and DNA replication in the silkworm, Bombyx mori. Insect Sci. 2022;29(4):1006-1016. doi:10.1111/1744-7917.12991(IF:3.262)
[6] Xiao Q, Dong ZQ, Zhu Y, et al. Bombyx mori Nucleopolyhedrovirus (BmNPV) Induces G2/M Arrest to Promote Viral Multiplication by Depleting BmCDK1. Insects. 2021;12(12):1098. Published 2021 Dec 8. doi:10.3390/insects12121098(IF:2.769)
[7] Chen Y, Tang J, Lu T, Liu F. CAPN1 promotes malignant behavior and erlotinib resistance mediated by phosphorylation of c-Met and PIK3R2 via degrading PTPN1 in lung adenocarcinoma. Thorac Cancer. 2020;11(7):1848-1860. doi:10.1111/1759-7714.13465(IF:2.610)
[8] Mao H, Zhang X, Yin L, Ji X, Huang C, Wu Q. Silencing of circ_0007299 suppresses proliferation, migration, and invasiveness and promotes apoptosis of ectopic endometrial stromal cells in endometriosis via miR-424-5p-dependent modulation of CREB1 [published online ahead of print, 2022 Jun 16]. Arch Gynecol Obstet. 2022;10.1007/s00404-022-06650-w. doi:10.1007/s00404-022-06650-w(IF:2.344)
Protein A/G琼脂糖或磁珠-抗体捕获神器,轻松搞定IP/COIP!
技术讲解
Ni-NTA和GST-tag琼脂糖磁珠 1小时快速纯化His和GST标签蛋白
应用方向
链霉亲和素及其衍生物
技术讲解
磁珠深度解析,真的非AMPure XP不可吗?
应用方向
链霉亲和素纯化介质——一品多用,使用灵活,你get到了吗?
技术讲解
Strep(Ⅱ)Sep Plus Agarose Purificat...
20795ES
Phenyl Agarose FF(HS) Phenyl(HS)强疏...
20480ES
Anti-His Affinity Gel Anti-His标签亲...
20589ES
rProtein A/G Agarose Resin 蛋白A/G琼脂...
36403ES
Geldex 30 PG高分辨琼脂糖凝胶层析填料(3-10 kDa)
20596ES
MBPSep Dextrin Agarose Resin 6FF M...
20515ES
Butyl Agarose 4FF(Butyl弱疏水层析介质)
20478ES
Phenyl Agarose FF(LS) Phenyl(LS)强疏...
20482ES