Welcome to BioReagent Hub Hotline:19920083827
产品资料来源于品牌方官网 yeasen.com  |  仅供选型参考,规格与库存以官方最新信息为准
品官方资料 · 来源可溯查看原始页面 ›
Vendor Tel:400-6111-883
Product data sheet PD-20507ES
GSTSep Glutathione Agarose Resin 谷胱甘肽琼脂糖树脂(用于GST标签蛋白纯化) GSTSep Glutathione Agarose Resin 谷胱甘肽琼脂糖树脂(用于GST标签蛋白纯化)
Enquiry

Tell us your volume and application; we reply with a quote.

Start enquiry

GSTSep Glutathione Agarose Resin 谷胱甘肽琼脂糖树脂(用于GST标签蛋白纯化)

Scan for product
亲和层析蛋白纯化
Product no.20507ES Sizes4 options Documents1 files SourceYeasen · Official
Cat. No. 20507ES10
现货 $888.00 促$928.00
Qty 1
Enquire now
Enquire / bulk Data sheet Compare

Specifications & Enquiry

Tick the specs you need to submit them together; or enquire one by one. Cat. numbers are carried into the RFQ.

Cat.No Spec Price Status
20507ES10 10 mL $888.00促$928.00 现货 Enquire
20507ES50 50 mL $3,658.00促$3,848.00 现货 Enquire
20507ES60 100 mL $6,558.00促$6,898.00 现货 Enquire
20507ES80 1000 mL $42,458.00促$48,686.00 现货 Enquire
Submit enquiry Price is on application; bulk sizes can be sampled on request.

Overview

Specifications

Brand翌圣生物 Yeasen
Cat.No20507ES
Specification基质(Matrix)6%交联的琼脂糖微球配体(Ligand)通过12原子间隔臂偶联的谷胱甘肽粒径(Bead size)45-165 µm载量(Capacity)>20 mg GST蛋白(40 kDa)/mL基质最大压力(PressureMa
Origin翌圣生物科技(上海)股份有限公司
技术平台 亲和层析
应用方法 蛋白纯化用于GST标签蛋白的纯化
生物特性参数 蛋白类型GST标签蛋白 表达/来源系统各种表达系统 应用GST标签蛋白纯化
技术参数 基质6%交联的琼脂糖微球 配体通过12原子间隔臂偶联的谷胱甘肽 粒径45-165 µm 载量>20 mg GST蛋白(40 kDa)/mL基质 最大压力0.1 MPa,1 bar pH稳定范围3-12 制剂配方含20%乙醇的1×PBS,凝胶和保护液比例1:1 储存缓冲液含20%乙醇的1×PBS 储存条件2~8℃保存,有效期2年
实验类型 亲和层析通过谷胱甘肽与GST标签特异性结合纯化目标蛋白 GST标签蛋白纯化用于纯化GST融合蛋白

FAQ

Q:样品和缓冲液可以直接使用吗?

A:所用水和 Buffer 在使用前最好用 0.22 µm 或 0.45 µm 滤膜过滤除菌。

Q:该填料可以重复使用吗?

A:可以。

Q:该产品能够使用多少次?

A:具体次数要根据实验结果反馈,如果保存和操作得当的话,一般可以使用次数>3。

Q:该产品的载量是多少?

A:>20 mg GST protein/mL 基质(40 kDa)。

Q:洗脱液怎么配?

A:按说明书用平衡缓冲液配制还原性谷胱甘肽(Cat#60342ES)即可,配完后需要检测pH是否跟平衡缓冲液一致,一般谷胱甘肽溶解后pH会偏酸,需重新调整到pH 7.4或pH 8.0(建议调整到pH 8.0,适当提高pH能提高洗脱效率)。

Q:做pull down对照也有条带?

A:可以调整下洗杂的缓冲液,1.补加氯化钠浓度至0.5 M左右,PBS溶液中氯化钠含量约0.15 M,可以再补加0.35 M左右。 2. PBS中添加终浓度0.1-0.2%Tween-20,通过去污剂作用减少蛋白非特异性吸附。 3. 洗杂步骤可以在清洗2-3次后将填料转移至新的EP管中继续清洗。

Documents & Citations

Published citations

  1. Cui Q, Bi H, Lv Z, et al. Diverse CMT2 neuropathies are linked to aberrant G3BP interactions in stress granules. Cell. 2023;186(4):803-820.e25. doi:10.1016/j.cell.2022.12.046(IF:64.5)
  2. Deng X, Xiao Y, Tang X, Liu B, Lin H. Arabidopsis α-Aurora kinase plays a role in cytokinesis through regulating MAP65-3 association with microtubules at phragmoplast midzone. Nat Commun. 2024;15(1):3779. Published 2024 May 6. doi:10.1038/s41467-024-48238-9(IF:16.6)
  3. Guo Y, Tan Y, Qu M, et al. OsWR2 recruits HDA704 to regulate the deacetylation of H4K8ac in the promoter of OsABI5 in response to drought stress. J Integr Plant Biol. 2023;65(7):1651-1669. doi:10.1111/jipb.13481(IF:11.4)
  4. Chen X, Duan Y, Qiao F, et al. A secreted fungal effector suppresses rice immunity through host histone hypoacetylation [published online ahead of print, 2022 May 20]. New Phytol. 2022;10.1111/nph.18265IF: 9.4 Q1 . doi:10.1111/nph.18265(IF:10.152)
  5. Jin Y, Pan Y, Zheng S, et al. Inactivation of EGLN3 hydroxylase facilitates Erk3 degradation via autophagy and impedes lung cancer growth. Oncogene. 2022;41(12):1752-1766. doi:10.1038/s41388-022-02203-2(IF:9.867)
  6. Yuan D, Li S, Shang Z, et al. Genus-level evolutionary relationships of FAR proteins reflect the diversity of lifestyles of free-living and parasitic nematodes. BMC Biol. 2021;19(1):178. Published 2021 Aug 30. doi:10.1186/s12915-021-01111-3(IF:7.431)
  7. Ma X, Yuan Y, Li C, et al. Brassinosteroids suppress ethylene-induced fruitlet abscission through LcBZR1/2-mediated transcriptional repression of LcACS1/4 and LcACO2/3 in litchi. Hortic Res. 2021;8(1):105. Published 2021 May 1. doi:10.1038/s41438-021-00540-z(IF:6.793)
  8. Yi JW, Wang Y, Ma XS, et al. LcERF2 modulates cell wall metabolism by directly targeting a UDP-glucose-4-epimerase gene to regulate pedicel development and fruit abscission of litchi. Plant J. 2021;106(3):801-816. doi:10.1111/tpj.15201(IF:6.486)
  9. Ma X, Yuan Y, Wu Q, Wang J, Li J, Zhao M. LcEIL2/3 are involved in fruitlet abscission via activating genes related to ethylene biosynthesis and cell wall remodeling in litchi. Plant J. 2020;103(4):1338-1350. doi:10.1111/tpj.14804(IF:6.141)
  10. Ma X, Ying P, He Z, Wu H, Li J, Zhao M. The LcKNAT1-LcEIL2/3 Regulatory Module Is Involved in Fruitlet Abscission in Litchi. Front Plant Sci. 2022;12:802016. Published 2022 Jan 21. doi:10.3389/fpls.2021.802016(IF:5.754)

Recommended reading

Related products

✉Email 💬Message 👤Member
↑Top