HisSep Ni-NTA Agarose Resin His标签蛋白琼脂糖纯化树脂

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亲和层析His标签蛋白纯化

Product no.20502ES Sizes4 options Documents1 files SourceYeasen · Official

Cat. No. 20502ES10

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Cat.No Spec Price Status

20502ES10 10 mL ¥648.00促~~¥678.00~~ 现货 Enquire

20502ES50 50 mL ¥2,708.00促~~¥2,848.00~~ 现货 Enquire

20502ES60 100 mL ¥5,048.00促~~¥5,328.00~~ 现货 Enquire

20502ES80 2× 500 mL ¥42,686.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No20502ES

Specification基质（Matrix）交联的6%琼脂糖凝胶粒径（Bead size）45-165 µm载量（Capacity）＞40 mg 6×His-tagged protein/mL 基质耐压（Tolerance Pressuremax）0.1 MPa,

Origin翌圣生物科技(上海)股份有限公司

技术平台 亲和层析

应用方法 His标签蛋白纯化从样品中分离纯化His标签蛋白

生物特性参数 蛋白类型亲和层析树脂（Ni-NTA琼脂糖树脂） 纯化标签His标签（6×His） 应用His标签蛋白纯化

技术参数基质交联的6%琼脂糖凝胶 配体NTA（氮川三乙酸） 螯合金属离子Ni2+ 粒径45-165 µm 载量>40 mg 6×His-tagged protein/mL 基质 耐压0.1 MPa 1 bar 储存缓冲液含20%乙醇的1×PBS 储存温度2~8℃ 有效期2年 凝胶和保护液比例1:1

实验类型 亲和层析实现His标签蛋白纯化的技术方法

FAQ

Q：柱子反压过高是什么原因？

A：填料可能被堵塞：裂解液中可能含微小的固体颗粒，建议上柱前使用滤膜（0.22µm 或 0.45µm）过滤，或离心去除。

Q：为什么洗脱组分中无目的蛋白？

A：蛋白可能是包涵体：可通过电泳检测裂解液，分析上清中是否有目的蛋白，包涵体蛋白需按照包涵体蛋白的纯化方式；

Q：洗脱组分不纯（含多种蛋白）是什么原因？

A：样品中含有其他 His 标签蛋白：通过调节 pH 值或咪唑浓度来优化洗杂条件。再使用其他纯化手段（如去离子交换，疏水等）进一步纯化洗脱组分。

Q：为什么填料颜色会变浅或变成白色？

A：镍离子脱落或者剥离，按照填料再生的操作重新挂镍离子。

Q：产品规格的体积指的是什么？

A：指的是填料的体积，不包括保护液的。

Q：填料流速慢的原因？

A：1.柱子在清洗平衡步骤流速减慢，柱子下筛板堵塞或者是试剂中有杂质堵塞。2.上样过程中流速逐渐降低，样品中有不溶物堵塞填料或者是样品粘稠。少数情况蛋白挂柱后流速也会减慢。 3.洗杂洗脱步骤流速减慢，蛋白不稳定在柱子上沉淀，可以选择低温条件纯化，或者是在整个纯化过程中样品试剂中加入甘油保护蛋白。

Q：说明书步骤的5-10倍柱体积具体指的的是什么？

A：指的是5-10倍的填料体积。

Documents & Citations

蛋白纯化产品目录 Open document

Published citations

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