Anti-DYKDDDDK (Flag) Affinity Gel (Secretory or Membrane Protein) Anti-Flag亲和纯化凝胶(分泌蛋白或膜蛋白）
勾选需要的规格可一次提交；也可以逐个询价。货号会一并带入询价单。
Q：假设蛋白要保持活性，用哪些物质洗脱？
A：可以使用非变性洗脱方式。
Q：该产品和进口厂家的比怎么样？
A：Yeasen 20585 蛋白结合量几乎是进口S*品牌 的 2 倍。
Q：用 flag 多肽进行洗脱，但是一直洗脱不下来？
A：介质的载量本身就不高，洗脱浓度有可能较低，有可能因浓度较低而看不到明显条带。建议下一步增加洗脱强度。
已发表文献
[1] Lin J, Jiang X, Dong M, et al. Hepatokine Pregnancy Zone Protein Governs the Diet-Induced Thermogenesis Through Activating Brown Adipose Tissue. Adv Sci (Weinh). 2021;8(21):e2101991. doi:10.1002/advs.202101991(IF:16.806)
[2] Han L, Zhang F, Liu Y, et al. Uterus globulin associated protein 1 (UGRP1) binds podoplanin (PDPN) to promote a novel inflammation pathway during Streptococcus pneumoniae infection. Clin Transl Med. 2022;12(6):e850. doi:10.1002/ctm2.850(IF:11.492)
[3] Chen X, Duan Y, Qiao F, et al. A secreted fungal effector suppresses rice immunity through host histone hypoacetylation [published online ahead of print, 2022 May 20]. New Phytol. 2022;10.1111/nph.18265. doi:10.1111/nph.18265(IF:10.152)
[4] Chen Y, Yang L, Lu Y, et al. Up-regulation of NMRK2 mediated by TFE3 fusions is the key for energy metabolism adaption of Xp11.2 translocation renal cell carcinoma. Cancer Lett. 2022;538:215689. doi:10.1016/j.canlet.2022.215689(IF:8.679)
[5] Chen X, Li X, Li P, et al. Comprehensive identification of lysine 2-hydroxyisobutyrylated proteins in Ustilaginoidea virens reveals the involvement of lysine 2-hydroxyisobutyrylation in fungal virulence. J Integr Plant Biol. 2021;63(2):409-425. doi:10.1111/jipb.13066(IF:7.061)
[6] Tang C, Zhou Y, Sun W, et al. Oncopeptide MBOP Encoded by LINC01234 Promotes Colorectal Cancer through MAPK Signaling Pathway. Cancers (Basel). 2022;14(9):2338. Published 2022 May 9. doi:10.3390/cancers14092338(IF:6.639)
[7] Zheng G, Jiang C, Li Y, et al. TMEM43-S358L mutation enhances NF-κB-TGFβ signal cascade in arrhythmogenic right ventricular dysplasia/cardiomyopathy. Protein Cell. 2019;10(2):104-119. doi:10.1007/s13238-018-0563-2(IF:6.228)
[8] Chen X, Tang J, Pei Z, et al. The 'pears and lemons' protein UvPal1 regulates development and virulence of Ustilaginoidea virens. Environ Microbiol. 2020;22(12):5414-5432. doi:10.1111/1462-2920.15284(IF:4.933)
Anti-Flag亲和纯化凝胶 ——IP/蛋白纯化好帮手
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