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Product data sheet PD-40743ES
MitoTracker® Deep Red FM 线粒体深红色荧光探针 MitoTracker® Deep Red FM 线粒体深红色荧光探针
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MitoTracker® Deep Red FM 线粒体深红色荧光探针

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线粒体研究双标实验
Product no.40743ES Sizes1 options SourceYeasen · Official
Cat. No. 40743ES50 现货 ¥449.00 促¥499.00
Qty 1
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Specifications & Enquiry

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Cat.No Spec Price Status
40743ES50 50 µg ¥449.00促¥499.00 现货 Enquire
Submit enquiry Price is on application; bulk sizes can be sampled on request.

Overview

Specifications

Brand翌圣生物 Yeasen
Cat.No40743ES
Specification50 µg
Origin翌圣生物科技(上海)股份有限公司
应用方法 线粒体研究研究线粒体形态、分布和功能 双标实验与绿色荧光探针联合用于多色标记分析
生物特性参数 细胞产品子类线粒体染色探针 细胞类型通用 应用线粒体研究
样本类型 细胞
技术参数 分子式C34H36Cl2N2 分子量543.58 外观Blue solid 激发波长644 nm 发射波长665 nm 储存条件-20℃避光干燥保存 运输条件室温运输 有效期1年 染料类型carbocyanine-based 官能团弱巯基反应性的氯甲基官能团
实验类型 线粒体染色特异性标记活性线粒体,用于荧光检测

FAQ

Q:染色只能看到在细胞核周围有红色的弥散状,看不到线粒体的结构?

A:可能是探针浓度太高或者探针孵育后没有清洗干净,造成有非特异性染色。

Q:活细胞内直接做线粒体染色的试剂有哪些?

A:40740、40741、40742、40743 都可以。

Q:没有 HBSS 用什么缓冲液,加多少体积上流式?

A:可以使用 PBS 或是无血清培养基替代,添加体积足够做流式即可 100-200ul。

Q:染色结果可以使用多功能酶标仪观察吗?

A:可以,最好是使用荧光显微镜或是共聚焦显微镜、流式仪器。

Q:线粒体探针的保质期是多久?

A:按规定标准储存,保质期一年。

Q:线粒体探针可以染植物、细菌吗?

A:该系列探针是针对动物细胞开发的,对于植物或是原核生物,由于存在细胞壁因而染色较为困难不是很方便,没有做过相关验证。

Q:线粒体探针染色后可以过夜在进行观察荧光吗?

A:该探针染色后应在 1h 内完成观察并拍照,过夜会导致荧光的消失,建议染色后立即进行观察以保证荧光拍照的结果。

Q:该线粒体探针染色后的固定剂是否可以更换为甲醇?

A:可以的。染色完成后,如需对细胞样本进行固定,除说明书建议外还可以使用预冷的100%甲醇于-20℃固定15min。

Documents & Citations

Published citations

[1] Fan XY, Guo L, Chen LN, et al. Reduction of mtDNA heteroplasmy in mitochondrial replacement therapy by inducing forced mitophagy. Nat Biomed Eng. 2022;6(4):339-350. doi:10.1038/s41551-022-00881-7(IF:25.671)

[2] Bai D, Du J, Bu X, et al. ALDOA maintains NLRP3 inflammasome activation by controlling AMPK activation. Autophagy. 2022;18(7):1673-1693. doi:10.1080/15548627.2021.1997051(IF:16.016)

[3] Ge X, Gao M, He B, et al. Rapid and high-throughput testing of antifungal susceptibility using an AIEgen-based analytical system [published online ahead of print, 2022 Jun 4]. Biomaterials. 2022;287:121618. doi:10.1016/j.biomaterials.2022.121618(IF:12.479)

[4] Liu C, Li L, Lyu J, et al. Split bullets loaded nanoparticles for amplified immunotherapy. J Control Release. 2022;347:199-210. doi:10.1016/j.jconrel.2022.05.011(IF:9.776)

[5] Liu K, Zhao Q, Liu P, et al. ATG3-dependent autophagy mediates mitochondrial homeostasis in pluripotency acquirement and maintenance. Autophagy. 2016;12(11):2000-2008. doi:10.1080/15548627.2016.1212786(IF:9.108)

[6] Liu K, Zhao Q, Sun H, et al. BNIP3 (BCL2 interacting protein 3) regulates pluripotency by modulating mitochondrial homeostasis via mitophagy. Cell Death Dis. 2022;13(4):334. Published 2022 Apr 11. doi:10.1038/s41419-022-04795-9(IF:8.469)

[7] Liu Z, Xia X, Lv X, Song E, Song Y. Iron-bearing nanoparticles trigger human umbilical vein endothelial cells ferroptotic responses by promoting intracellular iron level. Environ Pollut. 2021;287:117345. doi:10.1016/j.envpol.2021.117345(IF:8.071)

[8] Lin C , Yang X , Li H , et al. Self-assembled nanomedicine combining a berberine derivative and doxorubicin for enhanced antitumor and antimetastatic efficacy via mitochondrial pathways. Nanoscale. 2021;13(13):6605-6623. doi:10.1039/d1nr00032b(IF:7.790)

[9] Zhou C, Li J, Du J, et al. HMGCS1 drives drug-resistance in acute myeloid leukemia through endoplasmic reticulum-UPR-mitochondria axis. Biomed Pharmacother. 2021;137:111378. doi:10.1016/j.biopha.2021.111378(IF:6.530)

[10] Hu S, Huang B, Pu Y, et al. A thermally activated delayed fluorescence photosensitizer for photodynamic therapy of oral squamous cell carcinoma under low laser intensity. J Mater Chem B. 2021;9(28):5645-5655. doi:10.1039/d1tb00719j(IF:6.331)

[11] Wu H, Wang J, Ma H, Xiao Z, Dong X. MicroRNA-21 inhibits mitochondria-mediated apoptosis in keloid. Oncotarget. 2017;8(54):92914-92925. Published 2017 Oct 6. doi:10.18632/oncotarget.21656(IF:5.168)

[12] Guo X, Lu D, Zhang D, et al. Curved corannulene dually targets mitochondria and endoplasmic reticulum, and initiates apoptosis via localized ROS induction upon light triggering. Mater Sci Eng C Mater Biol Appl. 2020;106:110227. doi:10.1016/j.msec.2019.110227(IF:4.959)

[13] Lv H, Hu L, Xu J, Bo T, Wang W. Identification and functional analysis of the mitochondrial cysteine synthase TtCsa2 from Tetrahymena thermophila. J Cell Biochem. 2021;122(12):1817-1831. doi:10.1002/jcb.30136(IF:4.429)

[14] Zhang X, Yan Q, Mulatihan DN, et al. Pharmaceutical micelles featured with singlet oxygen-responsive cargo release and mitochondrial targeting for enhanced photodynamic therapy. Nanotechnology. 2018;29(25):255101. doi:10.1088/1361-6528/aabbdb(IF:3.404)

[15] Zhu Y, Wang T, He S, et al. Comparison of Antiobesity Effects of Adipose-Derived Stromal/Stem Cells from Different Sources in a Natural Aging Model [published correction appears in Diabetes Metab Syndr Obes. 2022 Mar 01;15:671-672]. Diabetes Metab Syndr Obes. 2021;14:4535-4546. Published 2021 Nov 15. doi:10.2147/DMSO.S334044(IF:3.168)

[16] Wang J, Wu H, Zhou Y, et al. HIF-1α inhibits mitochondria-mediated apoptosis and improves the survival of human adipose-derived stem cells in ischemic microenvironments. J Plast Reconstr Aesthet Surg. 2021;74(8):1908-1918. doi:10.1016/j.bjps.2020.11.041(IF:2.390)

[17] Yang K, Wang N, Guo HT, et al. Effect of L-carnitine on sperm quality during liquid storage of boar semen. Asian-Australas J Anim Sci. 2020;33(11):1763-1769. doi:10.5713/ajas.19.0455(IF:1.227)

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